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Volume 127, Issue 2, Pages (August 2004)

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1 Volume 127, Issue 2, Pages 524-534 (August 2004)
Nerve growth factor mediates alterations of colonic sensitivity and mucosal barrier induced by neonatal stress in rats  Frederick Barreau, Christel Cartier, Laurent Ferrier, Jean Fioramonti, Lionel Bueno  Gastroenterology  Volume 127, Issue 2, Pages (August 2004) DOI: /j.gastro

2 Figure 1 (A) Effect of neonatal treatment with active and inactivated anti-NGF antibodies on visceral sensitivity in 10-week-old rats previously submitted to neonatal MD (deprived rats) and (B) effect of neonatal NGF treatment in nondeprived rats. Values are means ± SEM (n = 10). ∗P < 0.05, significantly different from controls in the same graph; †P < 0.05, significantly different from corresponding nondeprived controls in B. Gastroenterology  , DOI: ( /j.gastro )

3 Figure 2 (A) Effect of neonatal treatment with anti-NGF antibodies on gut paracellular permeability in 12-week-old rats previously submitted to neonatal MD (deprived rats) and (B) effect of neonatal NGF treatment in nondeprived rats. Values are means ± SEM (n = 10). ∗P < 0.05, significantly different from controls in the same graph; †P < 0.05, significantly different from nondeprived controls in B. Gastroenterology  , DOI: ( /j.gastro )

4 Figure 3 (A) Effect of neonatal treatment with anti-NGF antibodies on colonic MPO activity in 12-week-old rats previously submitted to neonatal MD (deprived rats) and (B) effect of neonatal NGF treatment in nondeprived rats. Values are means ± SEM (n = 10). ∗P < 0.05, significantly different from controls in the same graph; †P < 0.05, significantly different from nondeprived controls in B. Gastroenterology  , DOI: ( /j.gastro )

5 Figure 4 Mast cell density on the last day of MD in (A) 14-day-old pups and (B) 12-week-old adult rats after anti-NGF treatment in deprived rats and (C) NGF treatment in nondeprived rats. Values are means ± SEM (n = 10). ∗P < 0.05, significantly different from controls in the same graph; †P < 0.05, significantly different from nondeprived controls in B. Gastroenterology  , DOI: ( /j.gastro )

6 Figure 5 Effects of neonatal MD on colonic NGF expression in 14-day-old pups and 12-week-old adult rats. NGF immunoreactivity (brown) in colonic sections of (A1 and A2) nondeprived and (B1 and B2) deprived rats at 14 days and (C1 and C2) nondeprived and (D1 and D2) deprived rats at 12 weeks. A1, B1, C1, and D1: bar = 100 μm; A2, B2, C2, and D2: bar = 50 μm. Gastroenterology  , DOI: ( /j.gastro )

7 Figure 6 Effects of (A) anti-NGF antibodies and (B) doxantrazole on rectal sensitivity of 12-week-old rats previously submitted to neonatal MD. Values are means ± SEM (n = 8). ∗P < 0.05, significantly different from nondeprived rats; †P < 0.05, significantly different from deprived rats. Gastroenterology  , DOI: ( /j.gastro )

8 Figure 7 Effects of (A) anti-NGF antibodies and (B) doxantrazole on gut paracellular permeability of 12-week-old rats previously submitted to neonatal MD. Values are means ± SEM (n = 8). ∗P < 0.05, significantly different from deprived rats treated with inactivated anti-NGF antibodies or doxantrazole vehicle. Gastroenterology  , DOI: ( /j.gastro )


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