1. Cyclic changes in expression of mRNA of vascular endothelial growth factor, its receptors Flt-1 and KDR/Flk-1, and Ets-1 in human corpora lutea 
Toshiaki Endo, M.D., Yoshimitsu Kitajima, M.D., Akira Nishikawa, M.D., Kengo Manase, M.D., Masabumi Shibuya, M.D., Ryuichi Kudo, M.D. 
Fertility and Sterility 
Volume 76, Issue 4, Pages (October 2001)
DOI: /S (01)02012-X

2. FIGURE 1
(A), Northern blot analysis of expression of mRNA of vascular endothelial growth factor (VEGF), KDR/Flk-1, Flt-1, and Ets-1 in human corpora lutea. Total RNA was extracted from luteal tissues of five patients in the early luteal phase (ELP), five in the mid-luteal phase (MLP), and five in the late luteal phase (LLP). Samples were transferred onto nylon membranes and hybridized with 32P-labeled cDNA probes of human VEGF, Flt-1, KDR/Flk-1 and Ets-1. L-38 was the control. (B), Density mRNA signals of VEGF, Flt-1, KDR/Flk-1, and Ets-1. All densities were normalized against the density of the L38 band. The density of each signal in the early luteal phase was the a control. ∗P<.05 vs. density in the early luteal phase or mid-luteal phase.
Endo.mRNA of VEGF and its receptors.Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)02012-X)

3. FIGURE 2
Immunohistochemical localization of VEGF, Flt-1, and KDR/Flk-1 in human corpus luteum in the early luteal phase, mid-luteal phase, and late luteal phase. (A), Immunostaining for VEGF in the early luteal phase. (B), Immunostaining for VEGF in the mid-luteal phase. (C), Immunostaining for VEGF in the late luteal phase. (D), Immunostaining for Flt-1 in the early luteal phase. (E), Immunostaining for Flt-1 in the mid-luteal phase. (F), Immunostaining for Flt-1 in the late luteal phase. (G) Immunostaining for KDR/Flk-1 in the early luteal phase. (H), Immunostaining for KDR/Flk-1 in the mid-luteal phase. (I), Immunostaining for KDR/Flk-1 in the late luteal phase. GL, granulosa lutein cell; TL, thecal lutein cell; DL, degenerated corpus luteum. Arrowheads indicate endothelial cells. Magnification, ×200.
Endo.mRNA of VEGF and its receptors.Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)02012-X)