1. Change in mRNA expression of Glasso network genes in differentiated PC12 cells following Caprin2 knockdown or Caprin2 overexpression.
Change in mRNA expression of Glasso network genes in differentiated PC12 cells following Caprin2 knockdown or Caprin2 overexpression. A, For knockdowm, NGF-treated PC12 cells were transduced with lentiviral vectors expressing either a Caprin2 shRNA (CaprinKD) or a scrambled shRNA (control). Relative mRNA expression of endogenous Caprin2 was examined by qRT-PCR. The relative mRNA level was calculated using 2^(−ΔΔct) method, where the expression level of control sample was calculated as 1. Error bar, SEM; ***p < 0.001; unpaired Student’s t test; n = 6. B, For overexpression, NGF-treated PC12 cells were transduced with adenoviral vectors expressing either Caprin2 (CaprinO) or eGFP (control). Relative mRNA expression of endogenous Caprin2 was examined by qRT-PCR. The relative mRNA level was calculated using 2^(−ΔΔct) method where the expression level of control sample was calculated as 1. Error bar, SEM; ***p < 0.001; unpaired Student’s t test; n = 4. C, NGF-treated PC12 cells were transduced with lentiviral vectors expressing either a Caprin2 shRNA (CaprinKD) or a scrambled shRNA (control). Relative mRNA expression of genes in the network was examined by qRT-PCR. The relative mRNA level was calculated using 2^(−ΔΔct) method where the expression level of control sample was calculated as 1. Error bar, SEM; *p < 0.05; **p < 0.01; ***p < 0.001; unpaired t test; n = 6. D, NGF-treated PC12 cells were transduced with adenoviral vectors expressing either Caprin2 (CaprinO) or eGFP (control). Relative mRNA expression of genes in the network was examined by qRT-PCR. The relative mRNA level was calculated using 2^(−ΔΔct) method where the expression level of control sample was calculated as 1. Error bar, SEM; *p < 0.05; **p < 0.01; ***p < 0.001; unpaired Student’s t test; n = 4.
Su-Yi Loh et al. eNeuro 2017;4:ENEURO
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