1. Analysis of miR‐1a‐3p target genes in the context of tumor‐induced hypersensitivity in the bone metastatic pain model QRT‐PCR analysis demonstrating induction of predicted miR‐1a‐3p target genes in lumbar DRGs in vivo following suppression of miR‐1a‐3p expression via inhibitor delivery intrathecally in vivo. * denotes p <: as compared to mismatch‐inhibitor group.QRT‐PCR analysis representing changes in expression of miR‐1a‐3p target genes in ipsilateral lumbar DRGs in vivo at day 8 in the bone metastases model. * denotes p = 0.041 as compared to sham group.Time course of tumor‐induced downregulation of Clcn3 expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.002 on PID‐4, on PID‐8, 0.05 on PID‐10 and 0.04 on PID‐15 as compared to sham group.Time course of tumor‐induced changes in miR‐1a‐3p expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.04 on PID‐4, on PID‐8, and 0.04 on PID‐10 as compared to sham group.In panels A, B, C &D statistical P value was calculated by ANOVA followed by post hoc Fischer's test, n = 3 mice per group.Immunohistochemical analysis of expression of Clcn3 protein in mouse DRG and colabeling with isolectin‐B4‐binding (IB4) non‐peptidergic nociceptors and peptidergic (CGRP‐positive) nociceptors.
Analysis of miR‐1a‐3p target genes in the context of tumor‐induced hypersensitivity in the bone metastatic pain model QRT‐PCR analysis demonstrating induction of predicted miR‐1a‐3p target genes in lumbar DRGs in vivo following suppression of miR‐1a‐3p expression via inhibitor delivery intrathecally in vivo. * denotes p <: as compared to mismatch‐inhibitor group.QRT‐PCR analysis representing changes in expression of miR‐1a‐3p target genes in ipsilateral lumbar DRGs in vivo at day 8 in the bone metastases model. * denotes p = 0.041 as compared to sham group.Time course of tumor‐induced downregulation of Clcn3 expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.002 on PID‐4, on PID‐8, 0.05 on PID‐10 and 0.04 on PID‐15 as compared to sham group.Time course of tumor‐induced changes in miR‐1a‐3p expression in ipsilateral lumbar DRGs via QRT‐PCR analysis. * denotes p = 0.04 on PID‐4, on PID‐8, and 0.04 on PID‐10 as compared to sham group.In panels A, B, C &D statistical P value was calculated by ANOVA followed by post hoc Fischer's test, n = 3 mice per group.Immunohistochemical analysis of expression of Clcn3 protein in mouse DRG and colabeling with isolectin‐B4‐binding (IB4) non‐peptidergic nociceptors and peptidergic (CGRP‐positive) nociceptors. Scale bars represent 50 µm.
Kiran Kumar Bali et al. EMBO Mol Med. 2013;5:
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