1. Volume 8, Issue 1, Pages 9-15 (January 2001)
Small molecule-based laser inactivation of inositol 1,4,5-trisphosphate receptor 
Takanari Inoue, Kazuya Kikuchi, Kenzo Hirose, Masamitsu Iino, Tetsuo Nagano 
Chemistry & Biology 
Volume 8, Issue 1, Pages 9-15 (January 2001)
DOI: /S (00)00051-X

2. Fig. 1
Structures of chromophore-conjugated IP3 (MGIP3) and its analogs.
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)

3. Fig. 2
Ca2+ release activity via IP3R induced by MGIP3 and its analogs. The averages of initial rates of Ca2+ release were plotted against the compound concentrations (IP3, diamonds, n≥3; MGIP3, filled circles, n≥3; 1L-MGIP3, open circles, n≥3; CMG, crosses, n≥1). Error bars represent S.E.M.
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)

4. Fig. 3
Structural difference between thiourea-linked MG and amide-linked MG.
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)

5. Fig. 4
IP3-induced Ca2+ release is inactivated by CALI using MGIP3. (a) Time courses of IICR before (black trace) and after (red trace) treatment with 1 μM MGIP3 and 635 nm laser irradiation for 3 min. Luminal Ca2+ concentration ([Ca2+]L) was monitored. Application of 1 μM IP3 is indicated by the horizontal bar. (b) Initial rates of IICR before (open columns) and after (filled columns) the indicated treatments (mean±S.E.M., the number of experiments is indicated at each column). *Significant differences were found only when the Ca2+ release rate of the double-treated group (red column) was compared with the other groups after treatment (P<0.0001, analysis of variance (ANOVA) test and Student’s unpaired t-test). The differences among the other three Ca2+ release rates after treatment were not statistically significant (P>0.6, ANOVA test). No significant difference was found among the Ca2+ release rates before treatment (P>0.9, ANOVA test).
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)

6. Fig. 5
Dependence of CALI on the period of laser irradiation and the concentration of MGIP3. (a) Specimens were subjected to 635 nm laser irradiation in the presence of 1 μM MGIP3 for the indicated period of time, and the ratio of the Ca2+ release rate after the treatment to that obtained before the treatment was plotted (filled circles, mean±S.E.M., n≥4). In control experiments specimens were subjected to the same solution change without laser irradiation (open circles, mean±S.E.M., n≥4). (b) Various concentrations of MGIP3 were applied to the specimen, and the relative Ca2+ release rates with (filled circles) or without (open circles) 3 min laser irradiation are shown (mean±S.E.M., n≥5).
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)

7. Fig. 6
Specificity of MGIP3-based CALI. CMG, 1L-MGIP3 or MGIP3 in the presence of 100 μM IP3 was added to the specimens followed by laser irradiation. The data of MGIP3 in Fig. 4b are also shown (red column). The concentration of the compounds was 1 μM and the laser irradiation time was 3 min throughout these experiments. Initial rates of Ca2+ release of each specimen before and after the indicated treatments are shown (mean±S.E.M., the number of experiments is indicated at each column). *Significant differences were found only when the Ca2+ release rate of the MGIP3-treated group (red column) was compared with the other groups after treatment (P<0.0005, ANOVA test and Student’s unpaired t-test). The differences among the other three Ca2+ release rates after treatment were not statistically significant (P>0.8, ANOVA test). No significant difference was found among the Ca2+ release rates before treatment (P>0.8, ANOVA test).
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)

8. Fig. 7
Effect of MGIP3-based CALI on the activity of SERCA. The Ca2+ loading rate of the permeabilized smooth muscle cells as an indicator of SERCA pump activity was evaluated from the slope of the normalized time course of Mg-ATP2−-induced Ca2+ loading. The Ca2+ loading rates of each specimen before and after the indicated treatments were measured, and the relative Ca2+ loading rates are shown (mean±S.E.M., the number of experiments is indicated at each column). No significant difference was found among all the relative Ca2+ loading rates (P>0.2, ANOVA test)
Chemistry & Biology 2001 8, 9-15DOI: ( /S (00)00051-X)