1. A new animal model for pulmonary hypertension based on the overexpression of a single gene, angiopoietin-1 
Danny Chu, MD, Christopher C Sullivan, MS, Lingling Du, MD, Augustine J Cho, BA, Masakuni Kido, MD, Paul L Wolf, MD, Matthew D Weitzman, PhD, Stuart W Jamieson, MB, FRCS, Patricia A Thistlethwaite, MD, PhD 
The Annals of Thoracic Surgery 
Volume 77, Issue 2, Pages (February 2004)
DOI: /S (03)01350-X

2. Fig 1
Constitutive angiopoietin-1 transgene mRNA expression by polymerase chain reaction methods (A) and protein expression by Western blot assay (B) in lungs of rodents injected with adeno-associated virus-angiopoietin-1 (AAV-Ang-1) compared with rodents injected with adeno-associated virus-lacZ (AAV-lacZ). Lung gene expression measured at 1 and 2 months after viral gene transfer. (Ang-1 = angiopoietin-1, bp = base pair, GAPDH = glyceraldehyde-3-phosphate dehydrogenase, kD = kilodalton.)
The Annals of Thoracic Surgery  , DOI: ( /S (03)01350-X)

3. Fig 2
(A) Photomicrograph of rodent lungs demonstrating severe hypertrophy/hyperplasia of medial layer of small arterioles measuring 40 μm compared with sham and control animals. (Bar = 25 μm.) (B) Photomicrograph of rodent lungs showing severe muscle hypertrophy of pulmonary arterioles measuring 300 μm compared with sham and control animals. (Bar = 150 μm.) (Hematoxylin & eosin stain, magnification ×40.) (Ang-1 = animals injected with adeno-associated virus-angiopoietin-1 [AAV-Ang-1]; lacZ = animals injected with adeno-associated virus-lacZ [AAV-lacZ]; Saline = animals injected with carrier saline.)
The Annals of Thoracic Surgery  , DOI: ( /S (03)01350-X)

4. Fig 3
Pulmonary artery pressures of rodents at (Top) 30 and (Bottom) 60 days postgene delivery demonstrating persistent significantly elevated pressures of adeno-associated virus-angiopoietin-1 (AAV-Ang-1) injected animals compared with control animals. (Ang-1 = animals injected with AAV-Ang-1; lacZ = animals injected with adeno-associated virus-lacZ (AAV-lacZ); mm Hg = millimeters of mercury; PAD = pulmonary artery diastolic pressure; PAS = pulmonary artery systolic pressure.)
The Annals of Thoracic Surgery  , DOI: ( /S (03)01350-X)

5. Fig 4
Whole lung gross Microfil pulmonary artery angiograms illustrate severe small vessel blunting in animals injected with adeno-associated virus-angiopoietin-1 (AAV-Ang-1) compared with control animals. (Left) Ang-1. (Right) lacZ. (Bar = 5 mm.) (Ang-1 = animal injected with AAV-Ang-1; lacZ = animal injected with adeno-associated virus-lacZ [AAV-lacZ].)
The Annals of Thoracic Surgery  , DOI: ( /S (03)01350-X)

6. Fig 5
In situ RNA hybridization photomicrograph shows viral-specific angiopoietin-1 transcript being expressed in the walls of pulmonary arterioles. (Left) Ang-1. (Right) Saline. (Bar = 25 μm, nitro blue tetrazolium/5-bromo-4-chloro-3-indolyl-phosphate stain, magnification 40×.)
The Annals of Thoracic Surgery  , DOI: ( /S (03)01350-X)