1. Spontaneous Formation of Two-Dimensional and Three-Dimensional Cholesterol Crystals in Single Hydrated Lipid Bilayers 
Roy Ziblat, Iael Fargion, Leslie Leiserowitz, Lia Addadi 
Biophysical Journal 
Volume 103, Issue 2, Pages (July 2012)
DOI: /j.bpj
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2. Figure 1
Bragg peaks of ceramide/cholesterol/POPC mixtures at varying ratios, from monolayers with surface pressure of 1.5 and 30 mN/m, and from bilayers deposited at 30 mN/m. The intensity of each plot was factorized for convenience of presentation.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2012 Biophysical Society Terms and Conditions

3. Figure 2
Deconvolution of Bragg peak components fitted by Voigt functions. Each peak is colored according to the phase it represents; ceramide – green, mixed Cer/Chol – blue, cholesterol rectangular phase – red, and cholesterol triclinic phase – black.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2012 Biophysical Society Terms and Conditions

4. Figure 3
Diffraction data from a 36:54:10 Cer/Chol/POPC bilayer. Top plot presents the peaks after deconvolution, colored according to phases: ceramide phase – green, mixed Cer/Chol phase – blue, cholesterol rectangular phase – red and cholesterol triclinic phase black. Each Bragg peak is labeled with Miller indices. The Bragg rods corresponding to each peak are given below, labeled and colored accordingly.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2012 Biophysical Society Terms and Conditions

5. Figure 4
2D to 3D nucleation by Bragg peaks obtained from bilayers of (A) 36:54:10 mol% Cer/:Chol/POPC 6 h after deposition, (B) 36:54:10 mol% Cer/Chol/POPC 1 day after deposition, and (C) 18:72:10 mol% Cer/Chol/POPC more than 1 day after deposition. Bragg peaks of the 2D rectangular phase decrease, whereas Bragg peaks of the 3D triclinic phase increase from A through B to C.
Biophysical Journal  , DOI: ( /j.bpj )
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6. Figure 5
AFM bioscope height images, measured in wet tapping mode. The compositions of the samples are specified in the figure as ratios of Cer/Chol/POPC. Each scan is 5 μm × 5 μm, and the z color range is between 0 and 25 nm. Images were linearly flattened. (A and B) Deposited using the WDL-LS technique. (C) Bilayers and tetralayers deposited by vesicle fusion. Lines on the AFM images represent the locations of height profile cuts that are given on the right side. In these profiles, 5 and 10 nm thick correspond to a bi- and tetralayer, respectively.
Biophysical Journal  , DOI: ( /j.bpj )
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7. Figure 6
Epifluorescence micrographs of antibody-bound bilayer. 24:36:40 mol% Cer/Chol/POPC labeled with MAb 58B1 at 7°C (A) and at 37°C (B); (C and D) 24:36:40 mol% Cer/Chol/POPC bilayers labeled with MAb 405F at the same temperatures. The sample at 7°C is inhomogeneous and displays two distinct phases, different in their fluorescence intensities. (C) Shows both types of regions. (E) 25:75 mol% Chol/POPC bilayer labeled with MAb 58B1 (positive control) and (F) with MAb 405F as a negative control, at 37°C. Antibody concentration is 1.5 μg/ml; exposure time for all micrographs is 9 s.
Biophysical Journal  , DOI: ( /j.bpj )
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8. Figure 7
Schematic representation of 40:60 Cer/Chol samples of monolayer at P = 1.5 and 30 mN/m, and a bilayer deposited at P = 30 mN/m. Crystalline domains are schematically illustrated colored according to phases: Ceramide phase, green; mixed ceramide/cholesterol phase, blue; cholesterol rectangular phase, red; and cholesterol triclinic phase, gray.
Biophysical Journal  , DOI: ( /j.bpj )
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9. Biophysical Journal 2012 103, 255-264DOI: (10.1016/j.bpj.2012.05.025)
Copyright © 2012 Biophysical Society Terms and Conditions