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Figure 4 Leukocyte subset isolation from brain tissue by enzymatic dissociation Leukocyte subset isolation from brain tissue by enzymatic dissociation.

Published byFerdinand Bader Modified over 5 years ago

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Presentation on theme: "Figure 4 Leukocyte subset isolation from brain tissue by enzymatic dissociation Leukocyte subset isolation from brain tissue by enzymatic dissociation."— Presentation transcript:

1 Figure 4 Leukocyte subset isolation from brain tissue by enzymatic dissociation
Leukocyte subset isolation from brain tissue by enzymatic dissociation (A) Accutase 15/papain 30 and Kit yielded the highest CD3+ T cells numbers, and the difference between accutase 15/papain 30 and papain 15 was significant. (B) There were no differences between any of the experimental groups regarding to CD4+ T cell numbers, or (C) CD8+ T cell numbers. (D) Enzymatic tissue dissociation with Kit offered the highest amounts of CD19+ B cells, and the increase in cell yield compared to accutase 15, papain 15, and papain 30 was significant. (E) Brain dissociation with Kit yielded a higher number of CD11b+ myeloid cells as compared to papain 15 and papain 30, and accutase 15/papain 30 treatment provided significantly higher cell numbers than papain 30. Groups were compared for normality by using the Kolmogorov-Smirnov test. Values were compared using the non-parametric test Mann-Whitney U test. *p < 0.05, **p < 0.01, ***p < Rehana Z. Hussain et al. Neurol Neuroimmunol Neuroinflamm 2018;5:e437 Copyright © 2018 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology.

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