1. Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics 
Jieqiang Lv, M.D., Xueqiong Zhu, M.D., Ph.D., Ke Dong, MS, Yi Lin, M.S., Yue Hu, M.D., Chundan Zhu, M.D. 
Fertility and Sterility 
Volume 90, Issue 5, Pages (November 2008)
DOI: /j.fertnstert
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
Two-dimensional electrophoresis map of normal myometrium (N) and uterine leiomyoma (T), using a pH 3–8 immobilized pH gradient linear strip in the first dimension and a 200 mm × 185 mm × 1.0 mm 12% sodium dodecyl sulfate–polyacrylamide gel in the second dimension. The protein loading was 150 μg, and the gel was stained with the Silver Stain Plus kit from Bio-Rad.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
Identification of gamma down-regulation in leiomyoma tissue. (A) One of the protein spots with a consistent more than fivefold down-regulation in the leiomyoma specimens (arrow). N = normal myometrium; T = uterine leiomyoma. (B) Mass spectrometry of in-gel trypsin digests of this protein and analysis of the depicted peptide spectrum resulted in the identification of gamma.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
Western blot analysis revealed markedly down-regulated gamma expression in leiomyoma samples (T) compared with that in normal (N) myometrium tissues. Depicted are 6 individual-matched pairs of normal myometrium and leiomyoma tissue samples.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Comparative gamma reverse transcriptase polymerase chain reaction (RT-PCR) of individual-matched normal myometrium (N) and leiomyoma (T) tissues. In line with the results at the protein level, the RT-PCR results revealed marked down-regulation of gamma in leiomyoma tissues compared with individual-matched normal myometrium tissue. Depicted are four individual- matched pairs of normal myometrium and leiomyoma tissue samples.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions