1. Students
Get handout - FRQs
LL mid-point Monday
Food & drinks off of tables
Phones in bin…muted or off…please & thank you

2. Electron transport chain
Figure How noncyclic electron flow during the light reactions generates ATP and NADPH
P700 +
CO2
Photosystem II (PS II)
H2O
Light
LIGHT REACTIONS
CALVIN CYCLE O2
NADPH
[CH2O] (sugar) e
Primary acceptor
2 H+
1⁄2 1
Energy of electrons Pq
Cytochrome
complex Pc
ATP
Electron transport chain
NADP+
Photosystem I (PS I) 6 2
ADP 5 Fd
Electron
Transport
chain 7
reductase
+ 2 H+ 8
+ H+ 4 e e O2 3
Light
P680 1

3. Lab 4: Photosynthesis Dye-reduction technique…..
-Tests that light & chloroplasts are needed for light reactions to occur
DPIP replaces NADP+ as the final electron acceptor
DPIP becomes when reduced (gaining electrons)
Color change can be measured with spectrophotometer
Experiment – write this summary down!!
Set wavelength to 605 – check spec filter
Prepare cuvettes from Table 4.3 (Don’t add chloroplasts….yet)
Blank spec – read instructions next to wavelength knob
Add chloroplasts & invert 2X to mix
Place in spec & record for time 0 minutes
Place in front of light
Record at 5 minute intervals
(get chloroplasts on ice please)
Lab write-up - due WEDNESDAY
just what is on the sheet….no pre-lab, purpose, procedure

4. Unboiled Chloroplasts, Dark 3 Unboiled, Chloroplasts, Light 4
Cuvettes 1
Blank 2
Unboiled Chloroplasts,
Dark 3
Unboiled, Chloroplasts,
Light 4
Boiled, Chloroplasts, 5 No
Chloroplasts
Phosphate Buffer
1 mL
dH2O
4 mL
3 mL
3 mL +
3 drops
DPIP
Unboiled Chloroplasts
_______
Boiled Chloroplasts
20 drops = 1 mL
Add chloroplasts LAST