1. In Vitro Expansion and Transplantation of Intestinal Crypt Stem Cells
Vivian S.W. Li, Hans Clevers 
Gastroenterology 
Volume 143, Issue 1, Pages (July 2012)
DOI: /j.gastro
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2. Figure 1
In vitro expansion of human colonic organoids from a single stem cell. Representative colon spheroid derived from a single EPHB2high human colonic crypt cell from day 0 to day 13. Scale bar, 50 μm.
(Reprinted with permission from Jung et al.23)
Gastroenterology  , 30-34DOI: ( /j.gastro )
Copyright © 2012 AGA Institute Terms and Conditions

3. Figure 2
Colon organoid transplantation. (A) Colon organoid cartoon with crypt and differentiated-domain. (B, C) Expanded organoids are introduced into the colon damaged by dextran sulphate sodium. (D) Transplanted organoids eventually fuse to cover the lesion. (E, F) Confocal image of long-term engrafted (6 months) clonal organoids derived from an RFP+ donor mouse into RFP− recipient mouse. Of note, transplanted organoids form complete colonic crypts with all cell lineages.24 Images are shown with (F) or without (E) 4′,6-diamidino-2-phenylindole staining.
(Courtesy of Mamoru Watanabe and Tetsuya Nakamura).
Gastroenterology  , 30-34DOI: ( /j.gastro )
Copyright © 2012 AGA Institute Terms and Conditions