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Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth.

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Presentation on theme: "Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth."— Presentation transcript:

1 Nicotine and cotinine stimulate secretion of basic fibroblast growth factor and affect expression of matrix metalloproteinases in cultured human smooth muscle cells  C.S. Carty, MD, P.D. Soloway, PhD, S. Kayastha, BA, J. Bauer, B. Marsan, MD, J.J. Ricotta, MD, M. Dryjski, MD, PhD  Journal of Vascular Surgery  Volume 24, Issue 6, Pages (December 1996) DOI: /S (96) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 bFGF found in conditioned medium of human SMCs after 24 hours of incubation with different concentrations of nicotine or cotinine dissolved in no-growth medium. At the time indicated in the figure, supernatants were collected and stored frozen until analyzed with ELISA kit. Values represent mean of four experiments, with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Temporal induction of CL-1 mRNA by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine in concentrations of 10-8 and 10-7 mol/L dissolved in albumin medium for times indicated (6 to 36 hours). Northern blot analysis (10 μg of total RNA per lane) was performed with CL-1 cDNA probe. Blots were analyzed by autoradiography and quantitated by phosphor-imaging techniques. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells (exposed to albumin medium). Values represent mean of two to four experiments with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 2 Temporal induction of CL-1 mRNA by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine in concentrations of 10-8 and 10-7 mol/L dissolved in albumin medium for times indicated (6 to 36 hours). Northern blot analysis (10 μg of total RNA per lane) was performed with CL-1 cDNA probe. Blots were analyzed by autoradiography and quantitated by phosphor-imaging techniques. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells (exposed to albumin medium). Values represent mean of two to four experiments with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 3 Temporal induction of STM-1 mRNA by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine in concentrations of 10-8 and 10-7 mol/L dissolved in albumin medium for times indicated (6 to 36 hours). Northern blot analysis (10 μg of total RNA per lane) was performed with STM-1 cDNA probe. Blots were analyzed by autoradiography and quantitated by phosphor-imaging techniques. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells (exposed to albumin medium). Values represent mean of two to four experiments with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6 Fig. 3 Temporal induction of STM-1 mRNA by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine in concentrations of 10-8 and 10-7 mol/L dissolved in albumin medium for times indicated (6 to 36 hours). Northern blot analysis (10 μg of total RNA per lane) was performed with STM-1 cDNA probe. Blots were analyzed by autoradiography and quantitated by phosphor-imaging techniques. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells (exposed to albumin medium). Values represent mean of two to four experiments with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7 Fig. 4 Northern blots of TPI and STM-1 mRNA expression induced by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine (10-8 mol/L) for 6, 12, 18, 24, and 36 hours. Each lane corresponds to one time point. Top rows represent TPI expression and bottom rows correspond to STM-1. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

8 Fig. 4 Northern blots of TPI and STM-1 mRNA expression induced by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine (10-8 mol/L) for 6, 12, 18, 24, and 36 hours. Each lane corresponds to one time point. Top rows represent TPI expression and bottom rows correspond to STM-1. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

9 Fig. 5 Temporal induction of gelatinase A mRNA by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine in concentrations of 10-8 and 10-7 mol/L dissolved in albumin medium for times indicated (6 to 36 hours). Northern blot analysis (10 μg of total RNA per lane) was performed with gelatinase A cDNA probe. Blots were analyzed by autoradiography and quantitated by phosphor-imaging techniques. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells (exposed to albumin medium). Values represents mean of two to four experiments with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

10 Fig. 5 Temporal induction of gelatinase A mRNA by nicotine (A) and cotinine (B). Human SMCs were exposed to nicotine or cotinine in concentrations of 10-8 and 10-7 mol/L dissolved in albumin medium for times indicated (6 to 36 hours). Northern blot analysis (10 μg of total RNA per lane) was performed with gelatinase A cDNA probe. Blots were analyzed by autoradiography and quantitated by phosphor-imaging techniques. mRNA levels were normalized with TPI probe and compared with levels in simultaneously analyzed control cells (exposed to albumin medium). Values represents mean of two to four experiments with SEM indicated by vertical bars. Journal of Vascular Surgery  , DOI: ( /S (96) ) Copyright © 1996 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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