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Takahiro Watanabe, Kunihiko Tamaki 

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1 Cidofovir Diphosphate Inhibits Molluscum Contagiosum Virus DNA Polymerase Activity 
Takahiro Watanabe, Kunihiko Tamaki  Journal of Investigative Dermatology  Volume 128, Issue 5, Pages (May 2008) DOI: /sj.jid Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Primary structure comparisons and expression of viral DNA polymerase genes. (a) Alignment of the deduced amino-acid sequences of MC39L (GenBank accession number U60315), CPXV75 (DQ066528), and HSV-1 UL30 (AB231455). Boldface letters indicate identical amino acids. Gray boxes highlight identical and conserved amino acids. Dots indicate gaps in the sequence to allow optimal alignment. Numbers on the right indicate the amino-acid position. (b) Rabbit reticulocyte lysates were programmed with mRNA in the presence of [35S]methionine and then visualized by SDS-PAGE. The apparent molecular weights of the three viral DNA polymerases were 116kDa (MCV and CPV) and 140kDa (HSV-1), consistent with the predicted sizes and corresponding to the sizes of expressed or purified polypeptides in previous studies (Dorsky and Crumpacker, 1988; Haffey et al., 1988; McDonald and Traktman, 1994). The luciferase gene was expressed as a positive control. The positions and masses of protein markers are on the left. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 CDVpp inhibits in vitro translated DNA polymerase. DNA polymerase inhibition assays were performed as described in the text at the noted CDVpp concentrations. One representative assay out of three is shown for each viral DNA polymerase. The rabbit reticulocyte lysates were programmed with (a) MC39L, (b) CPXV75, and (c) HSV-1 UL30 mRNA and used for DNA polymerase assays. (d) For E. coli DNA polymerase assay, Klenow fragment (Takara Bio, Shiga, Japan) was used instead of programmed lysates. Journal of Investigative Dermatology  , DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

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