1. Volume 111, Issue 12, Pages 2735-2746 (December 2016)
Hexagonal Patterning of the Insect Compound Eye: Facet Area Variation, Defects, and Disorder 
Sangwoo Kim, Justin J. Cassidy, Boyuan Yang, Richard W. Carthew, Sascha Hilgenfeldt 
Biophysical Journal 
Volume 111, Issue 12, Pages (December 2016)
DOI: /j.bpj
Copyright © 2016 Biophysical Society Terms and Conditions

2. Figure 1
(a) Scanning electron micrograph of an adult Drosophila compound eye. (b) Eye epithelia dissected from midpupal stage and imaged for Discs Large protein. The left sample is wild-type and the right sample is a Fz mutant. (Arrows) Body axis orientation relative to the eye epithelia. (c) Magnified image of a single ommatidium in which the optical section is taken through the apical domain of the epithelium. C, cone cells; P, primary pigment cells; S, secondary pigment cells; T, tertiary pigment cells; B, bristle group. (d) Scanning electron micrograph of an Fz mutant adult compound eye. (e) Example of topological defects in a Fz mutant, color-coded by number of neighbors n. (f) Topology diagram of the entire eye from a Fz mutant (left) and its Voronoi reconstruction based on ommatidial centroids from image analysis (right); the latter faithfully reproduces most defects in the original experimental image. (g) Probability distribution of ommatidial area for wild-type and Fz mutant; the latter shows a considerably greater width (larger cA value). The bar in (a) provides a scale for all photographic images and is 35 μm for (a) and (d), 40 μm for (b) and (f), 1.5 μm for (c), and 5.5 μm for (e). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions

3. Figure 2
(a) Dual lattice of centroid points with nearest-neighbor bonds, extracted from an experimental image of an Fz mutant eye. (b and c) The pair correlation function g2(r) of a wild-type sample (b) and an Fz mutant sample (c), showing stronger loss of correlation in the latter. Distances are normalized by the mean distance in the dual lattice s¯. (d) The sample average of the translational correlation function 〈gT(r)〉s, for all genotypes discussed in this work (symbols). The wild-type, Ras, and Sev retinas display a slow decay superimposed over a large-scale oscillation, while the Fz mutant shows rapid (exponential) loss of correlation over a scale of only a few ommatidial lengths. (Dashed line) Modeling gT from a lattice with appropriate systematic area variation predicts the decay of the wild-type, Ras, and Sev results, but cannot explain Fz (see Systematic Variation of Ommatidial Area for more detail). (e) The orientational correlation function g6(r) is sensitive to hexagonal order. While Fz mutants display significantly smaller values, the correlation does not decay to zero over the size of the eye and the decay is not exponential. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions

4. Figure 3
(a) Schematic figure of the regular triangular lattice and a displacement of one of its points. (b) Voronoi diagram for different strength α of Gaussian perturbation or centroid points. As the perturbation becomes larger, the domain gets more disordered (color code is as in Fig. 1 e). (c) Correlation of cn and cA obtained from the Voronoi simulation, compared with the experimental values for wild-type equivalents and mutants. Error bars are standard deviations. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions

5. Figure 4
(a) Spatial distribution of ommatidium area (color scale) for a wild-type eye, representative of cA,sy values given in Table 2. The long-range area variation is visible. (b) The area gradient axis (red, diagonal line), in comparison with the dorsal-ventral axis (green, vertical line). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions

6. Figure 5
(a) Result of a Voronoi simulation performed with systematic area variation only (cA,sy ≈ 0.23), and no statistical variation. Even at this high value of cA,sy, systematic area variation does not induce any defects. (b) Size-topology correlation for cases where both statistical variation and systematic variation are present; the correlation curve shifts to the right with cA,sy. (c) When the statistical area variation is isolated by subtracting the effect of systematic variation, all curves collapse. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions

7. Figure 6
(a) Image analysis for determining vc. The interfaces between neighboring ommatidia, ommatidial frames, and the cluster of cone cells are identified. (b) Schematic diagram of the distances used in the exclusion volume calculation. (c) cn-cA correlation. (Dashed line) Result of a simulation without the exclusion volume. (Black solid line) Simulation of the gradient with cA,sy = 0.1. (Green, dot-dashed line) Including exclusion volume but neglecting systematic area variation leads to similar effects as in plain Voronoi simulations (compare to Fig. 5). (d) cn-cA,st correlation after subtraction of systematic area variation. All modeling results are consistent and in agreement with experiment. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions

8. Figure 7
Comparison of order measures from experimental data (symbols) with simulation results (solid lines). (a and b) The features of the pair correlation functions g2(r) from Fig. 2, a and b are captured very well in the model. (c and d) The decay of the translational correlation functions gT(r) from experimental wild-type (c) and Fz (d) samples is well represented by the modeling results. (e) The orientational correlation function g6(r) of wild-type samples hardly decays in experiment or theory. (f) The initial decay of g6(r) in modeled Fz mutant samples agrees with experiment, but larger distances show stronger correlation than the experiments. Scalar order parameter values T and g6 are reported in (a), (b), (e), and (f). To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
Copyright © 2016 Biophysical Society Terms and Conditions