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Functional Enhancer Screening in Single Cells

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Presentation on theme: "Functional Enhancer Screening in Single Cells"— Presentation transcript:

1 Functional Enhancer Screening in Single Cells
Joris van Arensbergen, Bas van Steensel  Molecular Cell  Volume 66, Issue 2, Pages (April 2017) DOI: /j.molcel Copyright © 2017 Elsevier Inc. Terms and Conditions

2 Figure 1 Schematic Representation of the Mosaic-Seq Strategy
(A) In a population of cells, different sgRNAs repress different enhancers by the recruitment of dCas9-KRAB, in some instances leading to a reduction of expression of a target gene. The presence of different sgRNAs is traced by a barcode that is present in the same construct as the sgRNA. (B) By combining the data from hundreds of single-cell RNA-seq (scRNA-seq) profiles for each sgRNA, the effect of each enhancer on each gene can be assessed. Molecular Cell  , DOI: ( /j.molcel ) Copyright © 2017 Elsevier Inc. Terms and Conditions

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