Presentation is loading. Please wait.

Presentation is loading. Please wait.

Identification of a new cryptic lipid‐binding domain in Ecm25.

Published byNergis Emre Modified over 5 years ago

Similar presentations

Presentation on theme: "Identification of a new cryptic lipid‐binding domain in Ecm25."— Presentation transcript:

1 Identification of a new cryptic lipid‐binding domain in Ecm25.
Identification of a new cryptic lipid‐binding domain in Ecm25. (A) Structure‐based alignment of Ecm25 and five known CRAL/TRIO domain. Conserved hydrophobic sites are colored blue, polar sites are red, invariant residues are boxed, sites occupied by small residues are magenta and the ligand‐binding sites of the structures are colored gray. Secondary structure elements are numbered according to the canonical C‐terminal CRAL/TRIO domain structure of Sec14 (PDB:1aua), and grayed out when disrupted or absent in some of the structures. Sequences prefixed with ‘h’ are from human; all others are from S. cerevisiae. (B) The truncated Ecm25‐CRAL/TRIO binds to artificial membranes in flotation assays. Liposomes contain 1 mM 1‐palmitoyl‐2‐oleoyl‐snglycero‐3‐phosphocholine (POPC) and phosphatidylserine (PS), PtdIns or PtdIns(3,4,5)P3, as indicated (3%, molar). Ecm25‐CRAL/TRIO was analyzed by western blot. Oriol Gallego et al. Mol Syst Biol 2010;6:430 © as stated in the article, figure or figure legend

Download ppt "Identification of a new cryptic lipid‐binding domain in Ecm25."

Similar presentations

Tuning the response of the DPI

Tuning the response of the DPI
(A, B) Stereoscopic views of the P3 RNase MRP RNA domain in complex with the RNase MRP/RNase P protein components Pop6 and Pop7. (A, B) Stereoscopic views.

(A, B) Stereoscopic views of the P3 RNase MRP RNA domain in complex with the RNase MRP/RNase P protein components Pop6 and Pop7. (A, B) Stereoscopic views.
The RNA components of eukaryotic RNase P and RNase MRP have multiple common structural features including the P3 domain. The RNA components of eukaryotic.

The RNA components of eukaryotic RNase P and RNase MRP have multiple common structural features including the P3 domain. The RNA components of eukaryotic.
Overview of the experimental setting.

Overview of the experimental setting.
Most promoters preserve their relative activity levels across conditions. Most promoters preserve their relative activity levels across conditions. (A)

Most promoters preserve their relative activity levels across conditions. Most promoters preserve their relative activity levels across conditions. (A)
Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.

Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.
Motif detectability corresponds to the phylogenetic profile of the cognate transcription factor. Motif detectability corresponds to the phylogenetic profile.

Motif detectability corresponds to the phylogenetic profile of the cognate transcription factor. Motif detectability corresponds to the phylogenetic profile.
Multiple sequence alignment of the BAR domains from Amphiphysins and from the subset of BAR domain family members that were shown to bind to a small GTPase.

Multiple sequence alignment of the BAR domains from Amphiphysins and from the subset of BAR domain family members that were shown to bind to a small GTPase.
Deubiquitination of Lys63-Linkage by a CYLD UBP

Deubiquitination of Lys63-Linkage by a CYLD UBP
Structure‐based functional characterization of H

Structure‐based functional characterization of H
Supplemental Figure S1. Alignment of drimenol synthase amino acid sequences from P. hydropiper and V. officinalis. Amino acid sequences were aligned with.

Supplemental Figure S1. Alignment of drimenol synthase amino acid sequences from P. hydropiper and V. officinalis. Amino acid sequences were aligned with.
Structure of in‐frame deletions.

Structure of in‐frame deletions.
A complete functional map of UBE2I

A complete functional map of UBE2I
Volume 125, Issue 1, Pages (April 2006)

Volume 125, Issue 1, Pages (April 2006)
Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.

Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.
Hierarchical Binding of Cofactors to the AAA ATPase p97

Hierarchical Binding of Cofactors to the AAA ATPase p97
Volume 124, Issue 1, Pages (January 2006)

Volume 124, Issue 1, Pages (January 2006)
Deubiquitination of Lys63-Linkage by a CYLD UBP

Deubiquitination of Lys63-Linkage by a CYLD UBP
Solution Structure of the U11-48K CHHC Zinc-Finger Domain that Specifically Binds the 5′ Splice Site of U12-Type Introns  Henning Tidow, Antonina Andreeva,

Solution Structure of the U11-48K CHHC Zinc-Finger Domain that Specifically Binds the 5′ Splice Site of U12-Type Introns  Henning Tidow, Antonina Andreeva,
DBM‐mimetic binding of KY‐02061 on the Dvl PDZ domain

DBM‐mimetic binding of KY‐02061 on the Dvl PDZ domain

Similar presentations

Ads by Google