Presentation is loading. Please wait.

Presentation is loading. Please wait.

Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion  Stephan.

Published byBernt Månsson Modified over 5 years ago

Similar presentations

Presentation on theme: "Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion  Stephan."— Presentation transcript:

1 Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion  Stephan Scheurer, PhD, Iris Lauer, PhD, Kay Foetisch, PhD, Mar San Miguel Moncin, MD, Mechthild Retzek, Christina Hartz, MSc, Ernesto Enrique, MD, Jonas Lidholm, PhD, Anna Cistero-Bahima, MD, Stefan Vieths, PhD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 4, Pages (October 2004) DOI: /j.jaci Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 Cherry extract (50 μg total protein) was digested with pepsin, separated by SDS-PAGE, silver stained (A), and tested for IgE reactivity with a pool of cherry allergic patients' sera (B). Two hours without pepsin (1); 30 seconds (2); 1 minute (3); 15 minutes (4); 30 minutes (5); 1 hour (6); 2 hours (7); BSA without pepsin (8); BSA, 2 hours (9). ∗Cherry allergens. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Purified recombinant cherry allergens (Pru av 1–HisTag, Pru av 3–HisTag, Pru av 4; 2 μg each) were digested with pepsin (20 μL; as long as 2 hours). Analysis of IgE reactivity was performed with a pool of cherry allergic patients' sera (1:6 dilution) sensitized to Pru av 1, Pru av 3, or Pru av 4, respectively. Buffer control (C1, H2O; C2, HCl). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 CD spectra of rPru av 1 (A) and Pru av 3 (B) were recorded at different temperatures: 20°C, 40°C, 70°C, 100°C, and after cooling down to 20°C. The spectral range was 185 to 255 nm, and the scanning rate was 50 nm/min. Ten scans were accumulated. MRD, Magnetic relaxation dispersion. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Analysis of IgE reactivity of heat-processed foods containing cherry by using sera from 2 patients allergic to cherry with sensitization to Pru av 1 (patient 2) and additional sensitization to Pru av 3 (patient 5). Fresh sweet cherries from the market (1); cherry fruit juice, 35% fruit content (Rapp's, Karben, Germany) (2); preserved cherries, sweetened (Kaiserkirschen mit Stein, Erlenhof, REWE-Erlenhof, Germany) (3); cherry jam, 45% fruit content (Erlenhof) (4). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

6 Fig 5 SDS-PAGE and silver staining (left) and immunoblotting (right) of 2 commercial cherry SPT extracts (A and B). Right, Detection of Pru av 1 and Pru av 3 by specific IgG (mouse anti–Pru av 1, rabbit anti–Pru p 3) and specific IgE from patients allergic to cherry (patient [pt] 3 sensitized to Pru av 1; pt 13 sensitized to Pru av 3). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

7 Fig 6 Histamine release from passively sensitized basophils from a cherry LTP–sensitized patient (patient 12) (A) and a Pru av 1–sensitized patient (patient 9) (B), induced by cherry extract (CE) prepared from fresh fruits, CE digested by pepsin, and CE prepared from 3 processed food products containing cherries (1, cherry fruit juice; 2, preserved cherries; 3, cherry jam). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2004 American Academy of Allergy, Asthma and Immunology Terms and Conditions

Download ppt "Strong allergenicity of Pru av 3, the lipid transfer protein from cherry, is related to high stability against thermal processing and digestion  Stephan."

Similar presentations

Bischenberg, 23 rd September 2007The GA 2 LEN/EAACI Allergy School Effects of heat treatment and proteolytic enzymes on allergenicity Dr. Montserrat Fernández.

Bischenberg, 23 rd September 2007The GA 2 LEN/EAACI Allergy School Effects of heat treatment and proteolytic enzymes on allergenicity Dr. Montserrat Fernández.
Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity  Abena S. Amoah, MSc, Benedicta.

Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity  Abena S. Amoah, MSc, Benedicta.
Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,

Detection of novel occupational wood allergens in locust wood dust (Robinia pseudoacacia L.)  Sabine Kespohl, PhD, Rolf Merget, MD, Axel Overlack, MD,
Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS 

Peter Vadas, MD, PhD, Boris Perelman, PhD, Gary Liss, MD, MS 
Napins, 2S albumins, are major allergens in oilseed rape and turnip rape  Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali,

Napins, 2S albumins, are major allergens in oilseed rape and turnip rape  Tuija J. Puumalainen, MSc, Sanna Poikonen, MD, Annika Kotovuori, MSc, Kirsi Vaali,
Pru p 3 acts as a strong sensitizer for peanut allergy in Spain

Pru p 3 acts as a strong sensitizer for peanut allergy in Spain
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R

Kuan-Wei Chen, PhD, Katharina Blatt, MSc, Wayne R
Margaretha A. Faber, MD, Vito Sabato, MD, Chris H

Margaretha A. Faber, MD, Vito Sabato, MD, Chris H
Allergy to latex, avocado pear, and banana: Evidence for a 30 kd antigen in immunoblotting  François Lavaud, MD, Alain Prevost, MD, Claude Cossart, MD,

Allergy to latex, avocado pear, and banana: Evidence for a 30 kd antigen in immunoblotting  François Lavaud, MD, Alain Prevost, MD, Claude Cossart, MD,
Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,

Identification and isolation of a Fel d 1–like molecule as a major rabbit allergen  Christiane Hilger, PhD, Stéphanie Kler, MSc, Karthik Arumugam, PhD,
Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.

Exploring the repertoire of IgE-binding self-antigens associated with atopic eczema  Sabine Zeller, MSc, Claudio Rhyner, PhD, Norbert Meyer, MD, Peter.
Eosinophilic gastrointestinal disease suggestive of pathogenesis-related class 10 (PR- 10) protein allergy resolved after immunotherapy  Liliane De Swert,

Eosinophilic gastrointestinal disease suggestive of pathogenesis-related class 10 (PR- 10) protein allergy resolved after immunotherapy  Liliane De Swert,
Recombinant lipid transfer protein Cor a 8 from hazelnut

Recombinant lipid transfer protein Cor a 8 from hazelnut
Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 

Santa Jeremy Ono, BA, PhD, Mark B. Abelson, MD 
Gloria García-Casado, PhD, Jesús F

Gloria García-Casado, PhD, Jesús F
Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.

Cor a 1–reactive T cells and IgE are predominantly cross-reactive to Bet v 1 in patients with birch pollen–associated food allergy to hazelnut  Claudia.
Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,

Act d 12 and Act d 13: Two novel, masked, relevant allergens in kiwifruit seeds  Sofía Sirvent, PhD, Belén Cantó, BSc, Javier Cuesta-Herranz, MD, PhD,
Digestibility of allergens extracted from natural rubber latex and vegetable foods  Takeshi Yagami, PhDa, Yuji Haishima, PhDa, Akitada Nakamura, PhDa,

Digestibility of allergens extracted from natural rubber latex and vegetable foods  Takeshi Yagami, PhDa, Yuji Haishima, PhDa, Akitada Nakamura, PhDa,
Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil  Cristiana Alonzi, MD, Paolo Campi, MD, Francesco.

Diagnosing IgE-mediated hypersensitivity to sesame by an immediate-reading “contact test” with sesame oil  Cristiana Alonzi, MD, Paolo Campi, MD, Francesco.

Similar presentations

Ads by Google