1. Modes of Protein Movement that Lead to the Asymmetric Localization of Partner of Numb during Drosophila Neuroblast Division 
Bingwei Lu, Larry Ackerman, Lily Yeh Jan, Yuh-Nung Jan 
Molecular Cell 
Volume 4, Issue 6, Pages (December 1999)
DOI: /S (00)80218-X

2. Figure 1
The C Terminus of Pon Is Both Necessary and Sufficient for Its Asymmetric Localization
Transgenic embryos expressing one of the four Myc-tagged deletion constructs of Pon (diagrammed in [A]) were immunostained for the constructs with anti-Myc antibody (green) and for DNA with propidium iodide (red). The amino acids deleted in each construct are: Δ1 (25–222), Δ2 (225–368), Δ3 (370–492), Δ4 (494–672). Δ1 deletes the Numb-interacting domain, and Δ3 deletes the coiled-coil domain. Metaphase neuroblasts expressing Δ1 (B), Δ2 (C), Δ3 (D), and Δ4 (E) were shown. (F) A mitotic neuroblast from a transgenic embryo expressing a fusion between GFP and the C terminus of Pon. (G) A mitotic neuroblast from an embryo injected with Pon–GFP(FS) RNA and stained with anti-GFP antibody. Arrows in each panel mark the neuroblasts being examined. The apical side of the neuroblast is up and basal side down in this and all other figures.
Molecular Cell 1999 4, DOI: ( /S (00)80218-X)

3. Figure 2
In Vivo Visualization of Pon–GFP Localization Using Time Lapse Confocal Microscopy
A stage 9–10 embryo of the genotype V32 Gal4/+; UAS Pon–GFP/+ was examined by time lapse imaging. (A–O) show a neuroblast that is followed through the cell cycle. Each image is a representative of a Z series of optical sections, and consecutive images are approximately 1 min apart. Arrows in (M)–(O) mark the apical neuroblast daughter cells, which do not receive Pon–GFP and are partially out of focus. Arrowheads in (L) and (M) indicate the cleavage furrow.
Molecular Cell 1999 4, DOI: ( /S (00)80218-X)

4. Figure 3
Localization of Endogenous Pon during the Neuroblast Cell Cycle
Stage 9 and 10 wild-type embryos were fixed and stained with anti-Pon antibody (green) and propidium iodide (red, for DNA). Neuroblasts that are at early interphase (A), late interphase (B), prophase (C), metaphase (D), anaphase (E), and telophase (F) of the cell cycle are shown. Note that at interphase, endogenous Pon is distributed in the cytoplasm and is excluded from the nucleus.
Molecular Cell 1999 4, DOI: ( /S (00)80218-X)

5. Figure 4
Fluorescence Recovery after Photobleaching Demonstrating Movement of Pon–GFP
Neuroblasts from embryos of the genotype V32 Gal4/+; UAS Pon–GFP/+ were targeted at the basal (A–C), apical (D–F), or lateral (G–I) side of the cortex or the entire cortex (P–R) for photobleaching. For each neuroblast, images of that cell before photobleaching (A, D, G, and P), after photobleaching (B, E, H, and Q), and after recovery (C, F, I, and R) are shown. The dotted lines indicate the boundary between photobleached and nonbleached areas, and arrows mark the photobleached cortex. (J–L) show a neuroblast from a BDM-treated embryo, and (M–O) show a neuroblast from a latrunculin-treated embryo. (J and M), embryos that are before photobleaching; (K and N), embryos after photobleaching; and (L and O), embryos after recovery.
Molecular Cell 1999 4, DOI: ( /S (00)80218-X)

6. Figure 5
Effects of Actin and Myosin Inhibitors on Pon–GFP Localization
Stage 9 and 10 embryos of the genotype V32 Gal4/+; UAS Pon–GFP/+ were treated with latrunculin A (B), BDM (C), or mock treated with water (A), then fixed and stained with anti-GFP antibody (green) and propidium iodide (red). Arrows point to the mitotic neuroblasts under observation.
Molecular Cell 1999 4, DOI: ( /S (00)80218-X)

7. Figure 6
Pon–GFP Localization in string, pebble, and inscuteable Mutant Backgrounds
Stage 9 and 10 embryos of the following genotypes were examined: (A and B) V32 GAL4, UAS Pon–GFP/+; stg7B/stg7B; (C and D) V32 GAL4, UAS Pon–GFP/+; pblNR/pblNR; (E–H) V32 GAL4, inscP43/V32 GAL4, inscP43; UAS Pon–GFP/+. The time intervals between the two images in (A and B), (C and D), (E and F), and (G and H) are approximately 20 min, 10 min, 5 min, and 5 min, respectively. Arrows mark the neuroblasts being described.
Molecular Cell 1999 4, DOI: ( /S (00)80218-X)