1. Volume 123, Issue 6, Pages 1949-1961 (December 2002)
Differential localization of colitogenic Th1 and Th2 cells monospecific to a microflora- associated antigen in mice 
Masaru Yoshida, *,‡, Yasuhiko Shirai, *, Tomohiro Watanabe, *,‡, Masashi Yamori, *, Yoichiro Iwakura, §, Tsutomu Chiba, ‡, Toru Kita, *, Yoshio Wakatsuki, * 
Gastroenterology 
Volume 123, Issue 6, Pages (December 2002)
DOI: /gast
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2. Fig. 1
Immunogenicity of OVA expressed in E. coli. CD4 T cells were purified from Rag2−/−/OVA-23-3 mice and stimulated in culture containing OVA (■) purified from ECOVA or commercially available natural OVA (□), which were presented by syngeneic APC. The cultured cells were pulsed with [3H]-thymidine on day 3 for the last 12 hours. (A) Mean CPM ± SD are shown. BALB/c mice were given ECOVA or ECLacZ once a week for 1 month by using a gastric lavage tube. (B) OVA-specific serum IgG and (C) secretory IgA in the colonic contents from the mice were measured by enzyme-linked immunosorbent assay by using plates coated with natural OVA. The mean optical density value ± SD of each group (n = 6) are shown (*P < 0.05).
Gastroenterology  , DOI: ( /gast )
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3. Fig. 2
SCID mice were reconstituted with 1 × 107 of CD4 T cells obtained from Rag2−/−/OVA-23-3 mice, which showed gradual weight loss after inoculation with ECOVA (1 × 109 CFU) (○). Such weight loss was not seen in mice who received identical populations of CD4 T cells and were given ECLacZ (1 × 109 CFU) (■). The body weight of each mouse was measured at the indicated time-points and normalized by that measured on day 1. Results are shown by percentages of mean body weight and the SD in each group (n = 6). Those results shown are representative of 3 independent experiments.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

4. Fig. 3
Histopathology of the colonic tissue in SCID mice who received CD4 T cells from Rag2−/−/OVA-23-3 mice and were then given ECOVA. (A–D) H&E-stained paraffin sections of the ascending colon are shown. The SCID recipients with OVA-specific T cells were inoculated with (A) ECLacZ or (B–D) ECOVA. Mice were killed on (B) day 12 and (A, C, and D) day 20. (B) In the mice administered ECOVA, cellular infiltration began mainly from the preexisting lymphoid follicles (arrowheads) in the villi or at the base of villi in close proximity to the vessels. (C, D) Massive infiltration by mononuclear cells in the LP and submucosal layer, destruction of the crypt architecture (arrowhead), dilated intestinal gland with infiltrating leukocytes (arrow), loss of goblet cells, and remarkable infiltration by mononuclear and polymorphonuclear cells and eosinophils were seen in (D) mice inoculated with ECOVA.
Gastroenterology  , DOI: ( /gast )
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5. Fig. 4
Immunohistochemistry of the ascending colon in SCID recipients with OVA-specific T cells and inoculated with (A–D; ×200) ECLacZ or (E–H; ×200) ECOVA. (A, E) CD3, (B, F) CD11c, (C, G) F4/80 antigen, and (D, H) Ly6G antigen–positive cells were stained in the colon of SCID mice. (F) The inlet shows CD11c-positive cells in the epithelial cell layer (arrow), which overlay a follicular structure stained positive with the anti-CD11c antibody.
Gastroenterology  , DOI: ( /gast )
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6. Fig. 5
Time course of weight loss in mice and histopathology of the colon. Splenocytes of Rag2−/−/OVA-23-3 mice were transferred and the mice were inoculated with ECLacZ (■) and ECOVA (□). ECOVA also was inoculated after transfer of CD4 T cells from IL-4−/−/OVA-23-3 (●) or IFN-γ−/−/OVA-23-3 mice (○). The mean body weight percentage ± SD in each group (n = 5) was determined at the indicated time-points. (A) Results shown are representative of 3 independent experiments. (B, D) H&E-stained and (C, E) CD3-stained sections of the colon in SCID mice who received CD4 T cells from (B, C) IL-4−/−/OVA-23-3 mice or (D, E) IFN-γ−/−/OVA-23-3 mice. Three days after cell transfer, each mouse was inoculated with ECOVA. Histopathology results shown are from day 12. Lymph follicles are indicated by arrows. CD3-positive cells migrated in a diffuse manner in mice transplanted with (C) CD4 T cells from IL-4−/−/OVA-23-3 mice, whereas CD3-positive cells mostly were seen in the follicles of mice transplanted with (E) CD4 T cells from IFN-γ−/−/OVA-23-3 mice.
Gastroenterology  , DOI: ( /gast )
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7. Fig. 6
The time-course of weight loss and histopathology of the colon in SCID mice who received Th cells by transfer. OVA-specific Th1 cells were transferred to SCID mice and then they were inoculated with ECLACZ (●) or ECOVA (○). Mice also were inoculated with ECOVA after transfer of OVA-specific Th2 cells (■). Mean body weight percentage ± SD in each group (n = 5) was determined at the indicated time-points. (A) Results shown are representative of 2 independent experiments. (C, E) H&E-stained and (B, D, and F) CD3-stained sections of the colon in SCID mice who received OVA-specific (C, B, and D) Th1 cells or (E, F) Th2 cells. Three days after cell transfer, each mouse was inoculated with (B) ECLacZ or (C–F) ECOVA. Histopathology results shown are from day 18. (B) Arrowheads indicate cells stained positive to CD3. (E) Arrow shows an enlarged lymph follicle in the villi, which is a conspicuous feature of the colitis in mice with OVA-specific Th2 cells and challenged with ECOVA. (E, F) Infiltration by CD3-positive T cells in those enlarged follicles is indicated by an arrow. (C) For comparison, lymph follicles in the mice with Th1 cells also are indicated by an arrow.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions