1. Community diversity and metagenome depth interact to influence assembly quality.
Community diversity and metagenome depth interact to influence assembly quality. Five hundred and twenty‐two metagenomic assemblies from the Human Microbiome Project (HMP) are shown here to demonstrate the complex interaction of underlying microbial α‐diversity (x axis, diversity within a sample measured as species richness) and assembly quality (y axis). The latter was measured as the size of the smallest contig such that the cumulative length of longer contigs exceeds 4 Mbp, normalized by the total sequenced microbial nucleotide count (The Human Microbiome Project Consortium, 2012a). Communities from each of the seven available body sites are highlighted in different colors, with each point's area proportional to the total input nucleotides for assembly. Microbial composition, metagenome depth, and assembly approach (not shown) all interact to greatly influence the resulting assembly quality.
Nicola Segata et al. Mol Syst Biol 2013;9:666
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