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Repression of hspA2 messenger RNA in human testes with abnormal spermatogenesis
Weon-Young Son, Ching-Tack Han, Ph.D., Suh-Ha Hwang, Jae-Ho Lee, Seokjoong Kim, M.D., Young Chan Kim Fertility and Sterility Volume 73, Issue 6, Pages (June 2000) DOI: /S (00)
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FIGURE 1 Hematoxylin and eosin stains and immunohistochemistry (magnification, ×200). For hematoxylin and eosin stains, (A) shows testis of normal spermatogenesis, group 1; (B) shows testis of spermatocyte arrest, group 2; (C) shows testis of Sertoli cell–only syndrome, group 3. For immunohistochemistry of cAMP-responsive element modulator protein, (D) shows testis of normal spermatogenesis; (E) shows testis of spermatocyte arrest; (F) shows testis of Sertoli cell–only syndrome. Positive cAMP-responsive element modulator immunoreactivity is indicated by the arrows. Son. Repression of hspA2 mRNA. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (00) )
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FIGURE 2 Competitive RT-PCR analysis for hspA2 transcripts. (A), For internal control, GAPDH was used; lane M = molecular-weight markers; lane 1 = normal spermatogenesis; lane 2 = spermatogenic arrest; lane 3 = Sertoli cell–only syndrome; lane 4 = negative control. For competitive RT-PCR, (B) shows normal spermatogenesis, group 1; (C) shows spermatogenic arrest, group 2; (D) shows Sertoli cell–only syndrome, group 3. Son. Repression of hspA2 mRNA. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (00) )
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FIGURE 3 Relative levels of hspA2 mRNA expression in testes in the three groups. Group 1 = normal spermatogenesis; group 2 = spermatogenic arrest; group 3 = Sertoli cell–only syndrome. Son. Repression of hspA2 mRNA. Fertil Steril 2000. Fertility and Sterility , DOI: ( /S (00) )
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