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IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1  Christiane Hilger, PhD,

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Presentation on theme: "IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1  Christiane Hilger, PhD,"— Presentation transcript:

1 IgE-mediated anaphylaxis caused by bites of the pigeon tick Argas reflexus: Cloning and expression of the major allergen Arg r 1  Christiane Hilger, PhD, Jean-Claude Bessot, MD, Nicolas Hutt, MD, Fabienne Grigioni, BsC, Frédéric de Blay, MD, Gabrielle Pauli, MD, François Hentges, MD  Journal of Allergy and Clinical Immunology  Volume 115, Issue 3, Pages (March 2005) DOI: /j.jaci Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

2 Fig 1 IgE immunoblotting of Argas reflexus protein extracts. Lanes 1-13, patient sera; lane N, nonallergic control; lanes S1-S2, rabbit antisera. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

3 Fig 2 Amino acid sequence alignment of Arg r 1 and different proteins isolated from tick salivary glands. Percentage of identity (%I) and similarity (%S) are referred to Arg r 1. Blue, All amino acids are identical; yellow, more than half are identical or highly similar. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

4 Fig 3 Electrophoretic analysis of purified Arg r 1. Coomassie stained SDS-PAGE. Lane M, molecular weight marker; lane 1, total A reflexus protein extract; lane 2, purified rArg r 1; lane 3, purified nArg r 1. Samples were run under reducing (a) and nonreducing conditions (b). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

5 Fig 4 Detection of specific IgE to rArg r 1 in sera of patients allergic to Arg r 1 and controls. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2005 American Academy of Allergy, Asthma and Immunology Terms and Conditions

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