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A new murine model of pulmonary eosinophilic hypersensitivity: Contribution to experimental asthma  Ana Lúcia Pereira de Siqueira, BSc, Momtchilo Russo,

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Presentation on theme: "A new murine model of pulmonary eosinophilic hypersensitivity: Contribution to experimental asthma  Ana Lúcia Pereira de Siqueira, BSc, Momtchilo Russo,"— Presentation transcript:

1 A new murine model of pulmonary eosinophilic hypersensitivity: Contribution to experimental asthma 
Ana Lúcia Pereira de Siqueira, BSc, Momtchilo Russo, MD, PhD, Ana Angélica Steil, BSc, Sandra Facincone, BSc, Mario Mariano, DVM, PhD, Sonia Jancar, BSc, PhD  Journal of Allergy and Clinical Immunology  Volume 100, Issue 3, Pages (September 1997) DOI: /S (97) Copyright © 1997 Mosby, Inc. Terms and Conditions

2 Fig. 1 Cell infiltration into BAL of mice after stimulation with antigen. Groups of mice were immunized with OA-AL or with EWI or were nonimmunized (control). After 14 days, all groups received an intratracheal instillation of aggregated ovalbumin (600 μg), and 48 hours later, BAL was performed, and cells were counted. Data represent means ± SEM of four to nine animals per group. *p < 0.05 compared with control group. sdip < 0.05 compared with OA-AL group. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

3 Fig. 2 EPO in BAL (A) and lung tissue (B) of mice after antigen stimulation. Groups of mice were immunized with OA-AL or with EWI or were nonimmunized (control). After 14 days, all groups received an intratracheal instillation of aggregated ovalbumin (600 μg), and 48 hours later, EPO activity was determined. Data represent means ± SEM of four to six animals per group. *p < 0.05 compared with control and OA-AL groups. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

4 Fig. 2 EPO in BAL (A) and lung tissue (B) of mice after antigen stimulation. Groups of mice were immunized with OA-AL or with EWI or were nonimmunized (control). After 14 days, all groups received an intratracheal instillation of aggregated ovalbumin (600 μg), and 48 hours later, EPO activity was determined. Data represent means ± SEM of four to six animals per group. *p < 0.05 compared with control and OA-AL groups. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

5 Fig. 3 Time course of EPO activity in lung tissue. Mice were immunized with EWI and 14 days later received an intratracheal instillation of ovalbumin (600 μg). Animals were killed on indicated days after antigen challenge. Data represent means ± SEM of three to five animals per group. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

6 Fig. 4 Histopathologic analysis of mouse lungs immunized with EWI 48 hours (A, B, C) or 21 days (D) after intratracheal challenge with ovalbumin. A, Small bronchus showing typical morphologic features of bronchoconstriction and peribronchial infiltration of eosinophils and mononuclear cells with eosinophils infiltrating the epithelial layer (original magnification, ×40). B, Small bronchus with mucus plug, mucosal folding, and inflammatory cell infiltration (original magnification, ×25). C, Small bronchus showing degeneration of epithelial cell layer and shedding (original magnification, ×40). D, Lung parenchyma with infiltration of inflammatory cells and alveoli with increased air space characteristic of emphysema (original magnification, ×25). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

7 Fig. 4 Histopathologic analysis of mouse lungs immunized with EWI 48 hours (A, B, C) or 21 days (D) after intratracheal challenge with ovalbumin. A, Small bronchus showing typical morphologic features of bronchoconstriction and peribronchial infiltration of eosinophils and mononuclear cells with eosinophils infiltrating the epithelial layer (original magnification, ×40). B, Small bronchus with mucus plug, mucosal folding, and inflammatory cell infiltration (original magnification, ×25). C, Small bronchus showing degeneration of epithelial cell layer and shedding (original magnification, ×40). D, Lung parenchyma with infiltration of inflammatory cells and alveoli with increased air space characteristic of emphysema (original magnification, ×25). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

8 Fig. 4 Histopathologic analysis of mouse lungs immunized with EWI 48 hours (A, B, C) or 21 days (D) after intratracheal challenge with ovalbumin. A, Small bronchus showing typical morphologic features of bronchoconstriction and peribronchial infiltration of eosinophils and mononuclear cells with eosinophils infiltrating the epithelial layer (original magnification, ×40). B, Small bronchus with mucus plug, mucosal folding, and inflammatory cell infiltration (original magnification, ×25). C, Small bronchus showing degeneration of epithelial cell layer and shedding (original magnification, ×40). D, Lung parenchyma with infiltration of inflammatory cells and alveoli with increased air space characteristic of emphysema (original magnification, ×25). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

9 Fig. 4 Histopathologic analysis of mouse lungs immunized with EWI 48 hours (A, B, C) or 21 days (D) after intratracheal challenge with ovalbumin. A, Small bronchus showing typical morphologic features of bronchoconstriction and peribronchial infiltration of eosinophils and mononuclear cells with eosinophils infiltrating the epithelial layer (original magnification, ×40). B, Small bronchus with mucus plug, mucosal folding, and inflammatory cell infiltration (original magnification, ×25). C, Small bronchus showing degeneration of epithelial cell layer and shedding (original magnification, ×40). D, Lung parenchyma with infiltration of inflammatory cells and alveoli with increased air space characteristic of emphysema (original magnification, ×25). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

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