1. FMR1 and AKT/mTOR signalling pathways: potential functional interactions controlling folliculogenesis in human granulosa cells 
Julia Rehnitz, Diego D. Alcoba, Ilma S. Brum, Katrin Hinderhofer, Berthe Youness, Thomas Strowitzki, Peter H. Vogt 
Reproductive BioMedicine Online 
Volume 35, Issue 5, Pages (November 2017)
DOI: /j.rbmo
Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
COV434 cell numbers following their treatment with different concentrations of inhibitors. Untreated cells or cells treated only with dimethyl sulfoxide (DMSO) were used as controls. The first two points on the graphs represent cell numbers before the initiation of inhibitor treatment.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
Gene expression changes in human granulosa cells following treatment with recombinant FSH (rFSH). Changes in the expression levels of AKT (P = 0.05), mTOR (P = 0.015), S6K (P = 0.05) and FMR1 (P = 0.031), between controls and cells treated with 0.3 IU/ml of rFSH. *P ≤ 0.05, compared with the control. Normally distributed data are presented as bar diagrams (mean ± SE); all other data are presented as box-and-whisker plots (median and interquartile range).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
SK6 and FMR1 gene expression profiles in human granulosa cells, following the treatment of cells with rapamycin and MK. Fold changes, after the treatment of COV434 cells with MK and rapamycin, AKT and mTORC1 inhibitors, of S6K (P = and P = 0.016, respectively) and FMR1 (P = and P = 0.001, respectively) gene expression levels. *P ≤ 0.05, compared with the control.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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5. Figure 4
FMRP expression. FMRP expression level was after the treatment of cells with 0.3 IU/ml FSH or rapamycin (*P < 0.05, compared with the control). Please note different y-axis scales in the graphs.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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6. Figure 5
SK6 protein expression levels in human granulosa cells, following the treatments of cells with rapamycin. Fold change in SK6 levels compared with that in the control (P = ). *P ≤ 0.05, compared with the control.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions