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Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas  Qiulu Pan, William Pao, Marc.

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Presentation on theme: "Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas  Qiulu Pan, William Pao, Marc."— Presentation transcript:

1 Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas  Qiulu Pan, William Pao, Marc Ladanyi  The Journal of Molecular Diagnostics  Volume 7, Issue 3, Pages (August 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 EGFR exon 21 L858R mutation assay design showing primer locations and native and mutant Sau96I restriction enzyme sites. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 EGFR exon 19 deletion assay: examples of detection of 9-, 12-, 15-, and 18-bp deletions in EGFR exon 19. The variable relative heights of the deleted and germline peaks may reflect different proportions of tumor cells in these clinical tumor DNAs, or different levels of copy number alterations in the mutated EGFR allele. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

4 Figure 3 Sensitivity analysis of EGFR exon 19 deletion assay using serial dilutions of H1650 cell line DNA into normal DNA. Note that for dilutions of 25% and less the scale has been adjusted to highlight the mutant peak. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

5 Figure 4 EGFR exon 21 L858R mutation assay: examples of undigested, digested germline, and digested mutant PCR products. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

6 Figure 5 Sensitivity analysis of EGFR exon 21 L858R mutation assay using serial dilutions of H1975 cell line DNA into normal DNA. Note that for dilutions of 12.5% and less the scale has been adjusted to highlight the mutant peak. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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