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Induction of systemic immunologic tolerance to β-lactoglobulin by oral administration of a whey protein hydrolysate  Rodolphe Fritsché, PhD, Jean Jacques.

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Presentation on theme: "Induction of systemic immunologic tolerance to β-lactoglobulin by oral administration of a whey protein hydrolysate  Rodolphe Fritsché, PhD, Jean Jacques."— Presentation transcript:

1 Induction of systemic immunologic tolerance to β-lactoglobulin by oral administration of a whey protein hydrolysate  Rodolphe Fritsché, PhD, Jean Jacques Pahud, PhD, Sophie Pecquet, Andrea Pfeifer, PhD  Journal of Allergy and Clinical Immunology  Volume 100, Issue 2, Pages (August 1997) DOI: /S (97) Copyright © 1997 Mosby, Inc. Terms and Conditions

2 Fig. 1 Characterization of cow's milk formulas by SDS-PAGE. Lane 1, Molecular weight markers (at left of lane 1 molecular weights are indicated in kilodaltons); lane 2, ALFARE; lane 3, BEBA-HA; lane 4, NAN. All formulas were analyzed at a concentration of 0.8 mg of protein equivalent per 10 ml of solution applied. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

3 Fig. 2 Residual βLG-specific allergenicity in cow's milk formulas. This was tested by tritiated serotonin release from rat mast cells sensitized passively with rat IgE anti-βLG antibodies. Dose-dependent release curves obtained by triggering sensitized mast cells with βLG or the three formulas are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

4 Fig. 3 Antibody suppression by oral tolerance with cow's milk formulas. ELISA (log5) titers of IgE and IgG anti-βLG or anti-ovalbumin antibodies in sera from rats orally tolerized with NAN, BEBA-HA, ALFARE, or water. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

5 Fig. 4 Dose dependency of antibody suppression. ELISA (log5) titers of IgE anti-βLG or anti-ovalbumin antibodies in sera from rats orally tolerized with increasing doses (0 to 120 gm/L) of NAN or BEBA-HA. BLG, β-Lactoglobulin; OVA, ovalbumin. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

6 Fig. 5 In vitro suppression of mast cell response. Tritiated serotonin release from mast cells passively sensitized with sera of rats orally tolerized with NAN, BEBA-HA, ALFARE, or water. Triggering was done with βLG (0.01 to 1000 μg/ml). Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

7 Fig. 6 In vivo suppression of mast cell response. RMCPII release from intestinal mast cells of rats orally tolerized with NAN, BEBA-HA, or water. Serum levels of RMCPII after challenge with whey proteins are shown for three different experiments in which the same protocol was used. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

8 Fig. 7 Lymphocyte stimulation determined by tritiated thymidine incorporation. Lymph node lymphocytes from rats pre-fed with NAN, BEBA-HA, or ALFARE or from control rats (group given water only) were stimulated with βLG. Dose-dependent incorporation curves are shown. Journal of Allergy and Clinical Immunology  , DOI: ( /S (97) ) Copyright © 1997 Mosby, Inc. Terms and Conditions

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