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Selective Expression of Chemokine Monokine Induced by Interferon-γ in Alopecia Areata
Sandrine Benoit, Atiye Toksoy, Matthias Goebeler, Reinhard Gillitzer Journal of Investigative Dermatology Volume 121, Issue 4, Pages (October 2003) DOI: /j x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 1 Spatial distribution of chemokine mRNA expression in hair follicles of AA. (a–e, h, i) In situ hybridization with [35S]UTP-labeled MIG or MCP-1 antisense probes (f, g) immunohistochemistry for lymphocyte markers. (a, b) longitudinal sections; (c–i) transversal sections. (a, b, e) MIG mRNA is expressed in the perifollicular infiltrate and the dermal papilla. (f, g) Immunohistochemistry performed on serial sections of (e) shows CD3+(f) and CD8+lymphocytes (g) at sites of MIG expression. (c, d) MIG mRNA is expressed perifollicularly as well as in the medulla of the hair shaft. (h, i) MCP-1 mRNA is detectable in the inner root sheath. (a, c, e, h) Bright-field illumination; (b, d, i) dark-field illumination of corresponding sites, which allows better identification of silver grain signals. Bar, 100 μm. Journal of Investigative Dermatology , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions
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