1. Binding competition between B2′B3 and B3 fragments of TcdB.
Binding competition between B2′B3 and B3 fragments of TcdB. Bar graphs represent quantified flow cytometry data shown as mean fluorescence intensity (MFI). CHO-K1 cells were exposed to 100 nM Alexa Fluor 647-labeled TcdB1 B2′B3 or Alexa Fluor 647-labeled TcdB2 B2′B3 for 1 h at 37°C. (A, B) Exposures performed in the presence or absence of a 10-fold molar excess of unlabeled TcdB1 B2′B3 or TcdB2 B2′B3. (C, D) Exposures carried out with and without a 10-fold molar excess of unlabeled TcdB1 B3 or TcdB2 B3. *, P < 0.01; **, P <
Jonathan J. Hunt et al. mSphere 2017; doi: /mSphere