1. Glial cell line–derived neurotrophic factor (GDNF) and its receptors in human ovaries from fetuses, girls, and women 
Jacob Farhi, M.D., Asangla Ao, Ph.D., Benjamin Fisch, M.D., Ph.D., Xiao Yun Zhang, M.Sc., Roni Garor, M.Sc., Ronit Abir, Ph.D. 
Fertility and Sterility 
Volume 93, Issue 8, Pages (May 2010)
DOI: /j.fertnstert
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2. Figure 1
IMH photographs of GDNF protein expression. (A) GDNF protein staining and IMH staining of a human ovary from a 22-GW-old fetus. Note the primordial and primary follicles, with the red-brown staining indicating GDNF protein expression in the oocytes (full cytoplasmic without nuclear staining) and in a portion of the GCs and stroma cells. Original magnification ×400. (B) GDNF protein staining and IMH staining of a human ovary from a 22-year-old girl. Note the antral and primary follicles, with red-brown staining indicating GDNF expression in the oocytes (full cytoplasmic with nuclear staining) and in a portion of the GCs and stroma cells. Original magnification ×400. (C) Negative control for GDNF protein expression. Section of the same human ovary as in panel A. Note the primordial and primary follicles, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (D) Negative control for GDNF protein expression. Section of the same human ovary as in panel B. Note the antral follicle, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (E) Positive control for GDNF protein expression. Section of mouse brain. Note the red-brown staining in the sample. Original magnification ×400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3. Figure 2
IMH photographs of RET and RET 51 protein expression. (A) RET protein staining and IMH staining of a human ovary from a 33-GW-old fetus. Note the primordial and primary follicles, with the red-brown staining indicating RET expression in the oocytes (full cytoplasmic without nuclear staining) and in a portion of the stroma cells. Original magnification ×400. (B) RET 51 protein staining. Section of a human ovary from a 15-year-old girl. Note the primordial follicles with the staining in oocytes (full cytoplasmic with nuclear staining) and in a portion of the GCs and stroma cells. Original magnification ×400. (C) Negative control for RET protein staining. Section of the same human ovary as in panel A. Note the primordial and primary follicles, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (D) Negative control for RET 51 protein expression. Section of the same ovary as in panel B. Note the primordial and primary follicles, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (E) Positive control for RET 51 protein staining. Section of mouse small intestine. Note the red-brown staining in the sample. Original magnification ×400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

4. Figure 3
IMH and ISH photographs of GFR-α1 protein and mRNA expression. (A) GFR-α1 protein staining. Section of a human ovary from a 33-GW-old fetus. Note the primordial and primary follicles with oocyte staining (full cytoplasmic with nuclear staining) and expression in a portion of the GCs and stroma cells. Original magnification ×400. (B) GFR-α1 mRNA staining. Section of human ovary from 31-year-old woman. Note the primordial follicles with the staining in the oocytes (partial cytoplasmic with nuclear staining) and in a portion of the GCs and stroma cells. Original magnification ×400. (C) Negative control of GFR-α1 protein expression. Section of the same ovary as in panel A. Note the primordial and primary follicle, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (D) Negative control for GFR-α1 mRNA expression. Section of the same ovary as in panel B. Note the primordial follicle, the overall blue staining, and the lack of red-brown staining. Original magnification ×400. (E) ISH positive control for alpha tubulin expression. Section of an ovary from a 19-year-old woman. Note the secondary follicle, with the red-brown staining indicating expression in the oocytes (full cytoplasmic with nuclear staining) and in the GCs and stroma cells. Original magnification ×400.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

5. Figure 4
Representative RT-PCR gel illustrating GDNF gene and RET gene expression in fetal and adult ovaries. (A) GDNF isoform I and II (397 bp and 319 bp, respectively). (B) RET 51 (480 bp). (C) RET 9 (311 bp). (D) β-Actin (274 bp). Lane 1: 100 bp DNA ladder. Lane 2: Ovary from a 28-year-old woman. Lane 3: Ovary from a 21-year-old woman. Lane 4: Ovary from a 37-year-old woman. Lane 5: Ovary from a 35-GW-old fetus. Lane 6: In panel a (GDNF), ovary from a 35-GW-old fetus, and in panels b–d (RET 51, RET 9, and β-actin, respectively) ovary from a 25-GW-old normal fetus. Lane 7: In panel a (GDNF), pooled ovarian tissue from three 22- to 23-GW-old fetuses, and in panels b–d (RET 51, RET 9, and β-actin, respectively) pooled ovarian tissue from two 25-GW-old fetuses. Lane 8: Pooled ovarian tissue from three 22- to 23-GW-old fetuses. Lane 9: RT (−) control. Lane 10: Pooled ovarian tissue from three 22- to 23-GW-old fetuses.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions