Presentation is loading. Please wait.

Presentation is loading. Please wait.

Activation of Proteinase-Activated Receptor-2 by Human Kallikrein-Related Peptidases  Kristina Stefansson, Maria Brattsand, Dirk Roosterman, Cordula Kempkes,

Published byΊσις Ελευθερόπουλος Modified over 5 years ago

Similar presentations

Presentation on theme: "Activation of Proteinase-Activated Receptor-2 by Human Kallikrein-Related Peptidases  Kristina Stefansson, Maria Brattsand, Dirk Roosterman, Cordula Kempkes,"— Presentation transcript:

1 Activation of Proteinase-Activated Receptor-2 by Human Kallikrein-Related Peptidases 
Kristina Stefansson, Maria Brattsand, Dirk Roosterman, Cordula Kempkes, Georgeta Bocheva, Martin Steinhoff, Torbjörn Egelrud  Journal of Investigative Dermatology  Volume 128, Issue 1, Pages (January 2008) DOI: /sj.jid Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Preparations of recombinant human tissue KLK protein precursors and active enzymes. Coomassie brilliant blue stained SDS-PAGE. Journal of Investigative Dermatology  , 18-25DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Ca2+ responses to trypsin and KLK in KNRK-PAR2 cells. KNRK-PAR2 cells were treated with (a) 1μM trypsin (T), (b) 10μM KLK5EK, (c) 10μM KLK7EK, (d) 10μM KLK8, and (e) 1μM KLK14. Trypsin or KLK was added at indicated time points (arrows). If no response could be detected, the integrity of the cells was verified by addition of 100nM trypsin (T). Journal of Investigative Dermatology  , 18-25DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Dose response curves for Ca2+ mobilization in response to trypsin and KLK. (a) KNRK-PAR2 cells were treated with active trypsin (circles), KLK5EK (triangles), KLK7EK (crosses), KLK8 (diamonds) or KLK14 (squares). Means±SEM, n≥3 for trypsin, KLK5EK, and KLK14. For KLK7EK and KLK8, experiments were carried out in duplicate at 1μM, at lower concentrations single experiments were performed. *1: statistically significantly different from baseline (n=3, P=0.04); *2: statistically significantly different from baseline (n=4, P=0.003). (b) Human dermal microvascular endothelial cells (hDMEC) cells were treated with active trypsin (circles), KLK5EK (triangles), or KLK14 (squares) with concentrations as indicated in the figure. 100% Ca2+ corresponds to fluorescence ratio 340/380nm=2. Journal of Investigative Dermatology  , 18-25DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Confocal photomicrographs of KNRK-PAR2 cells stimulated with trypsin or KLK. KNRK-PAR2 cells were stimulated with KLK5EK, KLK7EK, KLK8, KLK14, or trypsin, all at 1μM. Negative control shows cells incubated in DMEM/0.1% BSA only. Cells incubated with rEK looked like cells in negative control (data not shown). Cells were stained with anti-Flag antibody toward extracellular Flag epitope and anti-HA antibody toward intracellular HA11 epitope. Merged: superimposition of images in the same row. Yellow color in superimposed images indicates no degradation of extracellular parts of PAR2. Journal of Investigative Dermatology  , 18-25DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Immunoreactivity of KLK14 and PAR2 in inflamed skin tissues. Immunoreactivity of KLK14 as well as PAR2 was found widely distributed in the upper squamous and granular layer in patients with inflammatory skin disorders. (a–d) Atopic dermatitis, (e–h) rosacea. Staining was performed with antisera specific for (a, b, e, f) KLK14 or (c, g) PAR2. (d, h) Negative controls. Inset size bars=50μM (b, f) or 100μM. Journal of Investigative Dermatology  , 18-25DOI: ( /sj.jid ) Copyright © 2008 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Activation of Proteinase-Activated Receptor-2 by Human Kallikrein-Related Peptidases  Kristina Stefansson, Maria Brattsand, Dirk Roosterman, Cordula Kempkes,"

Similar presentations

Rosacea as a Disease of Cathelicidins and Skin Innate Immunity

Rosacea as a Disease of Cathelicidins and Skin Innate Immunity
Stefanie Krenzer, Heike Peterziel, Cornelia Mauch, Sachiko I

Stefanie Krenzer, Heike Peterziel, Cornelia Mauch, Sachiko I
Selective Substrates and Inhibitors for Kallikrein-Related Peptidase 7 (KLK7) Shed Light on KLK Proteolytic Activity in the Stratum Corneum  Simon J.

Selective Substrates and Inhibitors for Kallikrein-Related Peptidase 7 (KLK7) Shed Light on KLK Proteolytic Activity in the Stratum Corneum  Simon J.
Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia- Influenced Cell Interaction  Eva Buentke, Lena C. Heffler, Annika.

Natural Killer and Dendritic Cell Contact in Lesional Atopic Dermatitis Skin –Malassezia- Influenced Cell Interaction  Eva Buentke, Lena C. Heffler, Annika.
Fibroblast Activation Protein: Differential Expression and Serine Protease Activity in Reactive Stromal Fibroblasts of Melanocytic Skin Tumors  Margit.

Fibroblast Activation Protein: Differential Expression and Serine Protease Activity in Reactive Stromal Fibroblasts of Melanocytic Skin Tumors  Margit.
Expression of Transglutaminase 5 in Normal and Pathologic Human Epidermis  Eleonora Candi, Sergio Oddi, Andrea Paradisi, Alessandro Terrinoni, Marco Ranalli,

Expression of Transglutaminase 5 in Normal and Pathologic Human Epidermis  Eleonora Candi, Sergio Oddi, Andrea Paradisi, Alessandro Terrinoni, Marco Ranalli,
Overexpression of Laminin-8 in Human Dermal Microvascular Endothelial Cells Promotes Angiogenesis-Related Functions  Jie Li, Lisa Zhou, Hoang T. Tran,

Overexpression of Laminin-8 in Human Dermal Microvascular Endothelial Cells Promotes Angiogenesis-Related Functions  Jie Li, Lisa Zhou, Hoang T. Tran,
Efalizumab Therapy for Atopic Dermatitis Causes Marked Increases in Circulating Effector Memory CD4+ T Cells That Express Cutaneous Lymphocyte Antigen 

Efalizumab Therapy for Atopic Dermatitis Causes Marked Increases in Circulating Effector Memory CD4+ T Cells That Express Cutaneous Lymphocyte Antigen 
A Proteolytic Cascade of Kallikreins in the Stratum Corneum

A Proteolytic Cascade of Kallikreins in the Stratum Corneum
IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.

IL-13-Stimulated Human Keratinocytes Preferentially Attract CD4+CCR4+ T cells: Possible Role in Atopic Dermatitis  Rahul Purwar, Thomas Werfel, Miriam.
Pathogenic Epitopes of Autoantibodies in Pemphigus Reside in the Amino-Terminal Adhesive Region of Desmogleins Which Are Unmasked by Proteolytic Processing.

Pathogenic Epitopes of Autoantibodies in Pemphigus Reside in the Amino-Terminal Adhesive Region of Desmogleins Which Are Unmasked by Proteolytic Processing.
Epitopes in the Linker Subdomain Region of Envoplakin Recognized by Autoantibodies in Paraneoplastic Pemphigus Patients  Bingxin Zhang, Rui Zheng, Jing.

Epitopes in the Linker Subdomain Region of Envoplakin Recognized by Autoantibodies in Paraneoplastic Pemphigus Patients  Bingxin Zhang, Rui Zheng, Jing.
Keratinocyte-Specific Mesotrypsin Contributes to the Desquamation Process via Kallikrein Activation and LEKTI Degradation  Masashi Miyai, Yuuko Matsumoto,

Keratinocyte-Specific Mesotrypsin Contributes to the Desquamation Process via Kallikrein Activation and LEKTI Degradation  Masashi Miyai, Yuuko Matsumoto,
Protease-Activated Receptor-2 (PAR-2) Expression in Human Fibroblasts is Regulated by Growth Factors and Extracellular Matrix  Barry L. Gruber, Mary J.

Protease-Activated Receptor-2 (PAR-2) Expression in Human Fibroblasts is Regulated by Growth Factors and Extracellular Matrix  Barry L. Gruber, Mary J.
Cytokine Expression is Downregulated by Collagen-Polyvinylpyrrolidone in Hypertrophic Scars1  Fernando E. Krötzsch-Gómez, Janette Furuzawa-Carballeda,

Cytokine Expression is Downregulated by Collagen-Polyvinylpyrrolidone in Hypertrophic Scars1  Fernando E. Krötzsch-Gómez, Janette Furuzawa-Carballeda,
Calcium Ion Gradients and Dynamics in Cultured Skin Slices of Rat Hindpaw in Response to Stimulation with ATP  Moe Tsutsumi, Sumiko Denda, Kaori Inoue,

Calcium Ion Gradients and Dynamics in Cultured Skin Slices of Rat Hindpaw in Response to Stimulation with ATP  Moe Tsutsumi, Sumiko Denda, Kaori Inoue,
Anna Celli, Debra Crumrine, Jason M. Meyer, Theodora M. Mauro 

Anna Celli, Debra Crumrine, Jason M. Meyer, Theodora M. Mauro 
Cross-Linking of SPINK6 by Transglutaminases Protects from Epidermal Proteases  Jan Fischer, Yulia Koblyakova, Ties Latendorf, Zhihong Wu, Ulf Meyer-Hoffert 

Cross-Linking of SPINK6 by Transglutaminases Protects from Epidermal Proteases  Jan Fischer, Yulia Koblyakova, Ties Latendorf, Zhihong Wu, Ulf Meyer-Hoffert 
Yasushi Hanakawa, Trevor Selwood, Denise Woo, Chenyan Lin, Norman M

Yasushi Hanakawa, Trevor Selwood, Denise Woo, Chenyan Lin, Norman M
Increased Expression of the Orphan Nuclear Receptor NURR1 in Psoriasis and Modulation following TNF-α Inhibition  Marina O'Kane, Trevor Markham, Alice.

Increased Expression of the Orphan Nuclear Receptor NURR1 in Psoriasis and Modulation following TNF-α Inhibition  Marina O'Kane, Trevor Markham, Alice.

Similar presentations

Ads by Google