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Alessandro Paciaroni, Stefania Cinelli, Giuseppe Onori 

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1 Effect of the Environment on the Protein Dynamical Transition: A Neutron Scattering Study 
Alessandro Paciaroni, Stefania Cinelli, Giuseppe Onori  Biophysical Journal  Volume 83, Issue 2, Pages (August 2002) DOI: /S (02) Copyright © 2002 The Biophysical Society Terms and Conditions

2 Figure 1 Normalized elastic neutron scattering intensity of lysozyme in pure glycerol versus Q2, at five selected temperatures: 100, 200, 240, 260, and 300K, from top to bottom. The lines correspond to fitting with Eq. 5. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

3 Figure 2 Normalized elastic neutron scattering intensity of lysozyme at 300K vs. Q2, for the different measured water contents: △, h=0. g D2O/g Lys; *h=0.1g D2O/g Lys; ■, h=0.2g D2O/g Lys; ○, h=0.35g D2O/g Lys; ●, h=0.42g D2O/g Lys; ▴, h=0.83g D2O/g Lys. In all of the following figures the same notation will be used. The lines correspond to the fitting with Eq. 5. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

4 Figure 3 Estimated lysozyme self-diffusion coefficients versus 1000/T, for different water contents. The dashed lines correspond to the linear Arrhenius trend. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

5 Figure 4 〈u2〉tot versus T, for all the measured water contents. The solid line represents the harmonic trend of Eq. 6. Dashed lines are linear fits to the high-temperature data. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

6 Figure 5 〈u2〉tot of lysozyme solvated with only glycerol compared with the mean square displacements of pure glycerol (□). These data were taken from Wuttke et al., 1995. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

7 Figure 6 Lysozyme Td (●) versus h, compared with the glass transition TG and the estimated dynamical phase transition TC temperatures (dashed line) of the corresponding aqueous solutions of glycerol. The solid line is a guide for the eye. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

8 Figure 7 Td of lysozyme in glycerol-water mixtures (●) and of lysozyme hydrated powders (○) versus h. The dashed line is a guide for the eye. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions

9 Figure 8 Increase of 〈u2〉tot as a function of h with respect to the anhydrous sample at 200K (△), 250K (●), and 300K (○) versus h. The solid lines are guides for the eye. Biophysical Journal  , DOI: ( /S (02) ) Copyright © 2002 The Biophysical Society Terms and Conditions


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