1. Survival and multiplication of Burkholderia cepacia within respiratory epithelial cells 
Joanne L. Tipper, Eileen Ingham, Jonathan H. Cove, Neil J. Todd, Kevin G. Kerr 
Clinical Microbiology and Infection 
Volume 4, Issue 8, Pages (August 1998)
DOI: /j tb00394.x
Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

2. Figure 1
Double immunofluorescence assay showing A549 respiratory epithelial cells infected with the clinical strain of B. cepacia, C1359 (×200 magnification). Extracellular bacteria are labeled with Texas Red-conjugated secondary antibodies and appear red-orange. Intracellular bacteria are labeled with FITC-conjugated secondary antibodies and appear green.
Clinical Microbiology and Infection 1998 4, DOI: ( /j tb00394.x)
Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

3. Figure 2
Double immunofluorescence assay showing A549 respiratory epithelial cells infected with the environmental strain of B. cepacia, NCTC (X200 magnification). The numbers of green intracellular bacteria are significantly reduced compared to B. cepacia C1359-infected A549 monolayers (Figure 1).
Clinical Microbiology and Infection 1998 4, DOI: ( /j tb00394.x)
Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

4. Figure 3
Double immunofluorescence assay showing A549 respiratory epithelial cells infected with E. coli strain C600 (X200 magnification). No intracellular green bacteria are present.
Clinical Microbiology and Infection 1998 4, DOI: ( /j tb00394.x)
Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

5. Figure 4
Infection of A549 respiratory epithelial cells with B. cepacia strains C1359, NCTC and NCTC 10743, and control strains P. aeruginosa PA01 and CFPA and E. coli C600. The results are expressed as log10 mean viable bacteria per well (mean of duplicate wells per time point)±95% confidence limits.
Clinical Microbiology and Infection 1998 4, DOI: ( /j tb00394.x)
Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions

6. Figure 5
Infection of A549 respiratory epithelial cells with B. cepacia (C1359, NCTC and NCTC 10743) and control strains (P. aeruginosa PA01 and CFPA and E. coli C600). The results are expressed as log10 mean viable bacteria per well ±95% confidence limits. ∗, mean CFU per well B. cepacia is significantly different (p<0.05) from mean CFU per well controls at T24; ∗∗, decrease in bacterial number is significantly different (p<0.05) from 4 h to 24 h (control strains).
Clinical Microbiology and Infection 1998 4, DOI: ( /j tb00394.x)
Copyright © 1998 European Society of Clinical Infectious Diseases Terms and Conditions