1. Characterization of FcεRI-bearing CD123+ blood dendritic cell antigen-2+ plasmacytoid dendritic cells in atopic dermatitis 
Natalija Novak, MD, Jean-Pierre Allam, MD, Tobias Hagemann, MD, Claudia Jenneck, MD, Sylvia Laffer, PhD, Rudolf Valenta, MD, Jarema Kochan, PhD, Thomas Bieber, MD, PhD 
Journal of Allergy and Clinical Immunology 
Volume 114, Issue 2, Pages (August 2004)
DOI: /j.jaci

2. Fig 1
Phenotypic differences of pDCs obtained from the peripheral blood of patients with AD and healthy controls. The percentage of surface expression on CD123+ BDCA-2+ pDCs of the peripheral blood from healthy control volunteers (white bars) and patients with AD (black bars) is depicted on the y-axis; n=10 for each group.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

3. Fig 2
Surface expression of the trimeric variant of FcεRI on pDCs is higher in patients with AD than in healthy, nonatopic volunteers. A, FcεRI surface expression and IgE binding is higher in patients with AD. RFI of the surface expression is depicted on the y-axis (n=17, controls; n=26, patients with AD). B, CD123+BDCA-2+ pDCs express enhanced intracellular amounts of the FcεRI-α chain and the FcεRI-γ chain (C). D, Transcripts of the FcεRIα-chain and FcεRIγ-chain are detectable on the RNA level of pDCs, whereas transcripts of the FcεRI-β chain are completely absent. This is 1 representative experiment of 3.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

4. Fig 3
Costimulatory molecules and MHC molecules are downregulated in consequence of FcεRI aggregation on pDCs of patients with AD. A, Surface expression of costimlatory molecules and MHC molecules on pDCs with FcεRI engagement via IgE and rabbit antihuman IgE (black bars/+) and unstimulated (white bars/Ø) is depicted on the y-axis. The percentage of BDCA-2+CD123+ pDCs expressing CLA (B) and CD62L (C) of nonatopic controls (white bars/Control) and patients with AD (black bars/AD) is depicted on the y-axis; n=17 nonatopic controls; n=18 patients with AD.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

5. Fig 4
Enhanced production of IL-10 after FcεRI engagement on pDCs. The intracellular amount of IL-10 produced by pDCs is enhanced 18 hours after stimulation of pDCs via FcεRI (B) in comparison with the unstimulated control (A). In parallel, the amount of IL-10 released in the supernatant by pDCs is significantly enhanced in consequence of FcεRI engagement via IgE and rabbit antihuman IgE (C); white bars/Ø=without FcεRI engagement; black bars/+=with FcεRI engagement; n=7. D, The amount of annexin V–positive pDC (depicted as percentage of annexin V+CD123+BDCA+ pDCs on the y-axis) of unstimulated (Ø/white bar) and FcεRI-activated pDC via IgE and rabbit antihuman IgE (+/black bar) after 18 hours of stimulation is shown; n=8.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

6. Fig 5
FcεRI stimulation of pDCs reduces their capacity to produce IFN-α and IFN-β and promotes TH2 immune responses. A, The amount of IL-4–producing T cells is enhanced after coculture of autologous naive cells with pDCs preloaded with allergen-specific IgE and recombinant allergen (black bars) in comparison with pDCs cocultured with naive T cells in the absence of allergens; n=12. The percentage of intracellular IFN-α (white bars) (B) and IFN-β (black bars) (C) produced in consequence of stimulation with CpG-A of pDCs with (+) and without (Ø) FcεRI preactivation by mAb against FcεRI (22E7) and goat-antimouse IgG (Fab′)2; n=15 experiments.
Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )