1. Volume 64, Issue 3, Pages 1125-1133 (September 2003)
The impact of immune-activating processes following transplantation on chronic allograft nephropathy 
Christoph Heidenhain, Anja Reutzel-Selke, Ulrike Bachmann, Sven Jonas, Andreas Pascher, Frank Ulrich, Johann Pratschke, Peter Neuhaus, Hans-Dieter Volk, Stefan G. Tullius 
Kidney International 
Volume 64, Issue 3, Pages (September 2003)
DOI: /j x
Copyright © 2003 International Society of Nephrology Terms and Conditions

2. Figure 1
Survival of renal allograft in recipients with and without further manipulations. All animals without further manipulations survived the observation period. In contrast, animals receiving additional skin grafts 4 and 8weeks after organ engraftment started dying 12weeks following organ engraftment. *P < 0.05; **P < 0.01 vs. control.
Kidney International  , DOI: ( /j x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

3. Figure 2
Allograft function following additional manipulations. Proteinuria did not increase in control animals; however. engraftment of third-party (A) or additional donor-specific (B) skin grafts resulted in a significant long-term increase of urinary protein excretion. *P < 0.05; **P < 0.01 vs. control; mean ± SEM.
Kidney International  , DOI: ( /j x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

4. Figure 3
Structural changes. Histology of renal allografts by the end of the observation period (16weeks) and those with additional manipulations (8weeks after the initial organ transplantation) (hematoxylin and eosin staining, ×250). Structural changes advanced significantly by week 16 following additional third-party and even more pronounced following donor-specific skin grafts performed 8weeks after the initial organ transplantation.
Kidney International  , DOI: ( /j x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

5. Figure 4
Cellular infiltrates. ED-1+ monocytes/macrophages (A) and CD4+ T cells (B) stained 16weeks after transplantation (magnification ×400). A significant increase of ED-1+ monocytes/ macrophages and CD4+ T cells was observed following both additional third-party and donor-specific skin grafts after an 8-week interval.
Kidney International  , DOI: ( /j x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

6. Figure 4
Cellular infiltrates. ED-1+ monocytes/macrophages (A) and CD4+ T cells (B) stained 16weeks after transplantation (magnification ×400). A significant increase of ED-1+ monocytes/ macrophages and CD4+ T cells was observed following both additional third-party and donor-specific skin grafts after an 8-week interval.
Kidney International  , DOI: ( /j x)
Copyright © 2003 International Society of Nephrology Terms and Conditions

7. Figure 5
Gene expression. CD25 mRNA was strongly up-regulated early (3days) and at the end of the observation period following third-party or donor-skin grafts performed 8weeks after the initial kidney transplantation. *P < 0.05; **P < 0.01 vs. control; mean ± SEM. RT-PCR is reverse transcription-polymerase chain reaction.
Kidney International  , DOI: ( /j x)
Copyright © 2003 International Society of Nephrology Terms and Conditions