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In vivo maturation of TH cells in relation to atopy

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1 In vivo maturation of TH cells in relation to atopy
Dominique M.A. Bullens, MD, PhD, Ahmad Kasran, MD, PhD, Ellen Dilissen, BSc, Kathleen De Swert, BSc, Lieve Coorevits, BSc, Jacques Van Snick, MD, PhD, Jan L. Ceuppens, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 128, Issue 1, Pages e7 (July 2011) DOI: /j.jaci Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Cytokine production by polyclonally activated CBMCs and PBMCs at the age of 12 and 24 months. CBMCs (5 × 105) obtained within 4 hours after birth or PBMCs (5 × 105) obtained at age 12 and 24 months were cultured with soluble anti-CD3 mAb (UCHT-1, 2 μg/mL) captured by murine P815/CD80 cells (1:1 ratio) in the absence (IL-9) or presence (IL-17 and IL-21) of the anti–IL-4 receptor mAb (2.5 μg/mL). Supernatants were collected after 3 days, and cytokine production was measured by means of ELISA. Box plots represent p10, median, and p90 values, and whiskers extend to minimum and maximum values. Allergic outcome was assessed at 2 and 6 years: E-A, early allergic; L-A, late allergic; N-A, not allergic. Journal of Allergy and Clinical Immunology  , e7DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Intracellular cytokines in CBMCs and purified CD4+ cord blood T cells
Intracellular cytokines in CBMCs and purified CD4+ cord blood T cells. Fresh CBMCs (left column) and purified cord blood CD4+ T cells (right column) from the same sample were stimulated with PHA, IL-23, and IL-1β. After 3 days, cells were restimulated with phorbol 12-myristate 13-acetate and ionomycin and membrane stained with anti-CD45 mAb (CBMCs only) and stained intracellularly with anti–IL-17–PE, anti–IL-21–PE, anti–IFN-γ–fluorescein isothiocyanate or IgG1–fluorescein isothiocyanate/IgG–PE control. Results are representative of 3 independent experiments. Journal of Allergy and Clinical Immunology  , e7DOI: ( /j.jaci ) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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