1. Automating SPE Extractions with the HT400E Systemby
William Amoyal
Disruptive Technologies

2. Disruptive Technologies history
Distribution company founded in 2005
Based in Villecresnes, France
Cover France, Belgium, Switzerland and other countries depending on product range
Agilent Service Partner for UV/Vis spectrophotometry, HPLC and CE
2 people
William Amoyal, Sales Manager
Franck Do Vale, Service Manager

3. Disruptive Technologies product range
Sample Preparation
Nucleic acides, protein and small molecules extraction from hard-to-lyse tissue samples
Online SPE system (couples with LCMS)
Dissolution/Formulation
Dissolution baths, friability and disintegration instruments
Physico Chemistry
HT Log P and pKa analyzer
HT oligonucleotides purity analyzer
HT proteins analyzer
HT DNA analyzer
HT Chiral analyzer
Kinetic solubility instruments
Service
Agilent Channel Partner to service their range of HPLC, UV Spectroscopy and CE
Rapid microbiology with Micro PRO
Analytical/preparative
OPLC chromatography solutions for semi preparative applications (OPLC systems, pumps, sample applicator, video imaging and densitometry instruments, reagent sprayer.
Spotter for MALDI and tissue MALDI imaging
ELSD detectors
Type-C silica hydride HPLC columns Flat sorbent beds for OPLC
Accessories and consumables for CE and HPLC
Validation kits for HPLC systems

4. Benefits of HT400E system Reliable Reproducible Flexible User friendly
Based on reliable technology of HTA autosampler already used by Agilent, Shimadzu, Perkin etc ... In their GC systems
Reproducible
Very low CV% inter-assay <2,5%
Flexible
Accepts many types of SPE cartridge, sample and target vials
User friendly
Intuitive “drag-and-drop” software
Competitive Price
Highest cost-to-performance SPE system available on the market

5. HT400E system with cooling unit

6. Main features of HT400E system
Can be used as an SPE system or HPLC autosampler
20/29 samples max per tray
4 solvents available (more on request – max 11)
Syringe and needle washing
Software manager QUICK-PREP
Sample tray Peltier cooling unit (optional)

7. Sample tray configurations
20/29 samples per TRAY
Sample volume: ∞ ml
SPE
1ml , 3ml standard
6 ml optional
Target Volume:
2 ml
4 ml
Syringe:
1 ml
2.5 ml
Other on request

8. Ideal workflow Cartridge activation Cartridge drying Sample loading
Volume, n. of solvent, n. of cycles
Cartridge drying
# of cycles
Sample loading
Volume, # of cycles
Sample washing
Volume, # of solvent, # of cycles
Elution
Volume, # of solvent, # of cycles, different target vials
Reagent addition
Volume, # of reagent, different target vials
Washing syringe and needle
Post-washing cartridge to re-use it

9. Easy-to-use Quick-Prep software
Drag-and-drop software
Tools such as Draw, Dispense, Mixing, Elution, Drying, Cartridge Picking, Cartridge Carry, Injection, Valve Washing, Cartridge Conditioning etc ... can be dragged and dropped in method making it possible to write methods in minutes
Easy and intuitive software
Easy set-up of the workstation (WorkTable)
Easy-write work-up process (WorkFlow)
Possibility to link methods

10. Application examples and results
The following evaluation has been made using the VMA kit of Chromsystems
The procedure has been as requested by Chromsystems protocol

11. Chromatogramme of VMA separation

12. Reproducibility of VMA separation
Retention Time
Area
Name
ISTD concentration
Units
2,685
VMA
4,670
mg/L
3,680
iso-VMA
0,000
10,478
5-HIAA
5,066
13,837
HVA
4,826 1 2 3 4 5 6 7 8
AVR.
S.D.
R.S.D
VMA
4,67
4,87
4,64
4,58
4,92
4,78
4,73
0,117
2,47%
5-HIAA
5,05
4,98
4,72
4,91
4,82
4,81
5,06
0,123
2,50%
HVA
4,83
4,66
4,74
4,75
0,083
1,75%

13. Carry-over on pathological control
Retention Time
Area
Name
ISTD concentration
Units
2,585
VMA
14,150
mg/L
3,500
iso_VMA
0,000
9,050
5-HIAA
15,390
12,172
HVA
12,711
Pathological Control

14. Carry-over on water WATER Retention Time Area Name ISTD concentration
Units
2,585
VMA
14,150
mg/L
3,500
iso_VMA
0,000
9,050
5-HIAA
15,390
12,172
HVA
12,711
WATER

15. Specifications HPLC Sample volume: Fixed loop Syringe sizes:
0.5ml, 1ml, 2.5ml, 10ml (2.5ml as standard)
Derivatization time:
User defined
Derivatization function:
Choice of reagents
Needle wash:
With solvent/sample
Loop wash:
HPLC tray option:
110 sample positions
SPE
SPE cartridge volumes available:
1ml, 3ml
Tray capacity:
29 tubes (20 as standard)
Tray temperature control option:
Peltier system. 10°C below ambient to +40°C
Solvent lines:
Up to 10 (4 as standard), plus one wash solvent
Physical Characteristics
Dimensions (W x H x D):
510 x 735 x 400mm
Mass:
15.0kg
Power:
110/220V AC, 50/60Hz

16. For more information on HT400E system
To see a video, click on picture besides in Diaporama mode or copy following URL in your browser
To download a brochure click on or copy following URL
HT400E SPE system brochure

17. Contact details at Disruptive Technologies
William Amoyal, Sales Manager
Disruptive Technologies
3 Allée des Camélias
94440 Villecresnes
Mobile: +33 (0)
Web: