Presentation is loading. Please wait.

Presentation is loading. Please wait.

Connective tissue growth factor expression is regulated by histamine in lung fibroblasts: Potential role of histamine in airway remodeling  Steffen Kunzmann,

Published byTorvald Jansen Modified over 5 years ago

Similar presentations

Presentation on theme: "Connective tissue growth factor expression is regulated by histamine in lung fibroblasts: Potential role of histamine in airway remodeling  Steffen Kunzmann,"— Presentation transcript:

1 Connective tissue growth factor expression is regulated by histamine in lung fibroblasts: Potential role of histamine in airway remodeling  Steffen Kunzmann, MD, Carsten Schmidt-Weber, PhD, Jean-Marc Zingg, PhD, Angelo Azzi, PhD, Boris W. Kramer, MD, Kurt Blaser, PhD, Cezmi A. Akdis, PhD, Christian P. Speer, MD  Journal of Allergy and Clinical Immunology  Volume 119, Issue 6, Pages (June 2007) DOI: /j.jaci Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Effect of histamine and TGF-β1 on the proliferation of lung fibroblasts. Proliferation was assessed 5 days after the addition of TGF-β1 (A) or histamine (B) at the indicated concentration. Values are means ± SEMs for 3 replicate cultures. ∗P < .05 compared with control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Histamine induces CTGF expression in lung fibroblasts on mRNA and protein level. A and B, Fibroblasts were stimulated with TGF-β1 and with histamine for the times indicated (A) or with different concentrations of histamine for 24 hours (B). Controls (c) represent untreated fibroblasts. CTGF mRNA expression was determined by semiquantitative competitive RT-PCR analysis (A) or by real-time RT-PCR (B). The upper panel shows electrophoretic visualization of amplicons, and the lower panel showsw GAPDH-normalized quantification of the PCR products (A). Data are means ± SEMs of 3 independent experiments. ∗P < .05 compared with control. C and D, Fibroblasts were stimulated with TGF-β1 or histamine. C shows the time dependency of TGF-β1 (left side) and histamine (right side) induction of CTGF protein expression. D shows the concentration dependency of histamine-induced CTGF protein expression (incubation time: 48 hours). Control (c) represents untreated fibroblasts. The upper panel shows the Western blots, and the lower panel shows the densitometric analysis of the relative increase of CTGF protein in cell lysats samples compared with control and normalized to β-actin. Three independent experiments were performed; representative blots are shown. ∗P < .05 compared with control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Histamine stimulates the CTGF promoter and the TGF-β–response element in the CTGF promoter. A and B, The CTGF promoter pCT-sb was transiently transfected into lung fibroblasts. Cells were then treated with TGF-β1 or/and histamine for 24 hours (A). B shows concentration dependency of histamine-induced pCT-sb-luc reporter activity. C and D, Luminometric analysis of AP-1- or TGF-β-RE-luc reporter transfected lung fibroblasts, incubated with TGF-β1 or histamine for 24 hours (C). D shows concentration dependency of histamine-induced TGF-β-RE-luc reporter activity. ∗P < .05 compared with control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 CTGF expression by histamine is mediated by HR1. A, The mRNA expression of the HR1-3 in lung fibroblasts was determined by RT-PCR analysis. The upper panel shows electrophoretic visualization of amplicons, and the lower panel shows GAPDH-normalized quantification of the PCR products. The bars represent the means of 3 independent experiments, including SEM. B, The effect of HR1 and HR2 antagonists on histamine-induced CTGF protein expression was studied by Western blotting. Lung fibroblasts were incubated with histamine, the HR1 antagonist triprolidine, the HR2 antagonist ranitidine, and histamine together with triprolidine or ranitidin for 48 hours. CTGF protein levels were determined by Western blotting. The upper panel shows the Western blots, and the lower panel shows densitometric analysis of the relative increase of CTGF protein in cell lysates samples compared with control and normalized to β-actin. Data are representative of 3 different experiments. ∗P < .05 compared with histamine alone. C and D, Luminometric analysis of CTGF promoter pCT-sb-luc transfected in lung fibroblasts and incubated with histamine for 24 hours in the presence of different concentrations of the HR1 antagonist triprolidine (C) or the HR2 antagonist ranitidin (D). ∗P < .05 compared with histamine alone. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig 5 TNF-α inhibits the induction of CTGF by histamine. A, Lung fibroblasts incubated with histamine or TNF-α and histamine together for 48 hours. Control (c) represents untreated fibroblasts. The upper panel shows the Western blots, and the lower panel shows densitometric analysis of the relative increase of CTGF protein in cell lysates samples compared with control and normalized to β-actin. All values were obtained from experiments performed in triplicate, and the error bars indicate SEMs. ∗P < .05 compared with histamine alone. B, Luminometric analysis of pCT-sb-luc or TGF-β-RE-luc reporter transfected lung fibroblasts, incubated with histamine or histamine and TNF-α. ∗P < .05 compared with histamine alone. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2007 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Download ppt "Connective tissue growth factor expression is regulated by histamine in lung fibroblasts: Potential role of histamine in airway remodeling  Steffen Kunzmann,"

Similar presentations

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.
Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components  Stephen R. Reeves, MD, PhD, Tessa.

Asthmatic airway epithelial cells differentially regulate fibroblast expression of extracellular matrix components  Stephen R. Reeves, MD, PhD, Tessa.
Immune deficiency caused by impaired expression of nuclear factor-κB essential modifier (NEMO) because of a mutation in the 5′ untranslated region of.

Immune deficiency caused by impaired expression of nuclear factor-κB essential modifier (NEMO) because of a mutation in the 5′ untranslated region of.
Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma  Kyung S. Lee, MS, Sun M. Jin,

Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma  Kyung S. Lee, MS, Sun M. Jin,
Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 

Induction of anergic allergen-specific suppressor T cells using tolerogenic dendritic cells derived from children with allergies to house dust mites 
Low E-prostanoid 2 receptor levels and deficient induction of the IL-1β/IL-1 type I receptor/COX-2 pathway: Vicious circle in patients with aspirin-exacerbated.

Low E-prostanoid 2 receptor levels and deficient induction of the IL-1β/IL-1 type I receptor/COX-2 pathway: Vicious circle in patients with aspirin-exacerbated.
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor  Akihiko Muto, PhDa, Sumiko.

Roles of the cytoplasmic domains of the α and β subunits of human granulocyte- macrophage colony-stimulating factor receptor  Akihiko Muto, PhDa, Sumiko.
Decreased serum vitamin D levels in children with asthma are associated with increased corticosteroid use  Daniel A. Searing, MD, Yong Zhang, PhD, James.

Decreased serum vitamin D levels in children with asthma are associated with increased corticosteroid use  Daniel A. Searing, MD, Yong Zhang, PhD, James.
Epigenetic regulation of established human type 1 versus type 2 cytokine responses  Ruey-Chyi Su, PhD, Allan B. Becker, MD, Anita L. Kozyrskyj, PhD, Kent.

Epigenetic regulation of established human type 1 versus type 2 cytokine responses  Ruey-Chyi Su, PhD, Allan B. Becker, MD, Anita L. Kozyrskyj, PhD, Kent.
Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,

Bruton tyrosine kinase mediates TLR9-dependent human dendritic cell activation  Vassilios Lougaris, MD, Manuela Baronio, PhD, Massimiliano Vitali, PhD,
IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,

IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis  Norbert Meyer, MD, Maya Zimmermann, PhD,
Induction of B7-H1 and B7-DC expression on airway epithelial cells by the Toll-like receptor 3 agonist double-stranded RNA and human rhinovirus infection:

Induction of B7-H1 and B7-DC expression on airway epithelial cells by the Toll-like receptor 3 agonist double-stranded RNA and human rhinovirus infection:
Histamine H2 receptor stimulation upregulates TH2 chemokine CCL17 production in human M2a macrophages  Susanne Mommert, PhD, Karl Gregor, MD, Kristine.

Histamine H2 receptor stimulation upregulates TH2 chemokine CCL17 production in human M2a macrophages  Susanne Mommert, PhD, Karl Gregor, MD, Kristine.
Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H

Julie M. Caldwell, PhD, Carine Blanchard, PhD, Margaret H
NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,

NF-κBp65-specific siRNA inhibits expression of genes of COX-2, NOS-2 and MMP-9 in rat IL-1β-induced and TNF-α-induced chondrocytes  Dr C. Lianxu, Ph.D.,
Deficient glucocorticoid induction of anti-inflammatory genes in nasal polyp fibroblasts of asthmatic patients with and without aspirin intolerance  Laura.

Deficient glucocorticoid induction of anti-inflammatory genes in nasal polyp fibroblasts of asthmatic patients with and without aspirin intolerance  Laura.
Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes  N. Chabane, M.Sc.,

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes  N. Chabane, M.Sc.,
Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.
Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3  Se Jin Park,

Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3  Se Jin Park,

Similar presentations

Ads by Google