1. The C-terminal part of FD binds to a G-box in the SEP3 promoter in vitro.
The C-terminal part of FD binds to a G-box in the SEP3 promoter in vitro. A, EMSA of the wild-type form of FD-C in combination with ν, FT, and TFL1. B, EMSA using the phosphomimic version of FD-C (FD-C_T282E) in combination with ν, FT, and TFL1. First lanes to the left in (A) and (B) show the free probe without any added proteins. Plus signs (+) above the lanes = which proteins were used in a specific EMSA reaction. Asterisk (*) = higher order complexes.
Silvio Collani et al. Plant Physiol. 2019;180:
©2019 by American Society of Plant Biologists