1. Genomic DNA in human blastocoele fluid
S. Palini, L. Galluzzi, S. De Stefani, M. Bianchi, D. Wells, M. Magnani, C. Bulletti 
Reproductive BioMedicine Online 
Volume 26, Issue 6, Pages (June 2013)
DOI: /j.rbmo
Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Calibration curves generated for estimating gene content. TBC1D3 (a) and TSPY1 (b) target sequences were amplified using 10-fold dilutions (5–0.005ng/reaction tube) of human male genomic DNA. Each dilution was amplified in triplicate. The curve equations and R2 values are presented on each panel. The calculated amplification efficiencies were 95% and 98% for TBC1D3 and TSPY1, respectively.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

3. Figure 2
Blastocoele fluid aspiration. Expanded day-5 blastocysts were removed from culture and transferred to a 25μl droplet of blastocyst medium under a paraffin oil underlay. The blastocysts were immobilized by a holding pipette connected to an oil-filled syringe and mounted on a micromanipulator. A finely pulled, oil-filled pipette was introduced through the mural trophectoderm to avoid damaging the inner cell mass and blastocoele fluid was aspirated gently until the blastocyst had fully collapsed around the pipette.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions