1. Supplemental Figure S4 a b c d Time after release from
Asyn R0
R30
R45
R60
R90
R120
Time after release from
nocodazole treatment (min)
R75 2N 4N
DNA Contents
Asyn R0
R30
R45
R60
R75
R90
R120
Time after release from
nocodazole treatment (min)
HAT activity
0.0
0.1
0.2
0.3
0.4
0.5 b H3
H2B
H2A H4
Asyn
2.5 5 6 7 8
8.5 9
9.5 10
10.5 11 12 13
Time after release from Thy-DB (h)
P-H3
Actin
H3AcK9
H3AcK14 12 13 11 10
10.5 9
9.5
8.5 2N 7 8 4N 5 6
2.5
Time after release
from Thy-DB (h)
DNA Contents
Asyn c
DNA
aAcK
Pericentrin
Merge G1
G1/S
(Thy DB) G2
(Eto)
Pro
NEBD
Meta
Early
Ana
Late
G2/M
Mitosis d
DNA Tubulin aAcK Merge
Prometaphase
Metaphase
Anaphase
Con shRNA
p300 shRNA