3. .1Sources of DNA and Sequencing Methods
2 Genome Assembly Strategy and Characterization
3 Gene Prediction and Annotation
4 Genome Structure
5 Genome Evolution
6 A Genome-Wide Examination of Sequence Variations
7 An Overview of the Predicted Protein- Coding Genes in the Human Genome
8 Conclusions

16. The Human Genome (Feb. 2001)
2.91 billion bp (Cellera) 3.2 billion (Hcon)
14.8 billions were sequenced in 9 months
5.1 times overlap 8 times in genes
26,588 (sure) genes for proteins genes for RNAs
+ ~12,000 sequences related to mouse etc.
1.1 % exons % introns 75% intergenic
About 50 % repeat sequences
45% transposables elements
Average length of a gene 27,894 bases
Exon ~100 bp , most: 234 in titin mRNA
Intron ~100-30,000 bp
2.1 million SNPs, less than 1% of them in proteins
Differences between human genomes 1 per 1250 bp

22. Nucleic acid enzyme 13%
Kinase 4%
Unknown 36%
hydrolase
isomerase