1. R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus
R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus A, BShown are HeLa cells stained for transfected R1441G‐LRRK2 (red) and either GFP‐Rab10 (A) or GFP‐Rab8 (B) in green. Scale bar, 10 μm. The second row in both panels shows enlarged portions boxed in the rows above. Scale bar, 2 μm.C, DPercent co‐localization of R1441G‐LRRK2 with the indicated Rab in the indicated cell regions was determined from a Mander's coefficient after automatic thresholding. Error bars represent mean ± SEM. ****P < 0.0001; **P =  by Student's unpaired, two‐tailed t‐test (n = 13 from two experiments). For peripheral quantitation, boxes of the size indicated were generated (see A) that included the plasma membrane and excluded the nucleus. For perinuclear quantitation (see B), the boxes included half of the nucleus.
Elena Purlyte et al. EMBO J. 2018;37:1-18
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