1. Dr. Thaweesak Tirawatnapong Chula Medical Research Center (Chula MRC)
New Technologies and Innovations in Laboratory Support of HIV/AIDS care and Treatment
Dr. Thaweesak Tirawatnapong
Chula Medical Research Center (Chula MRC)

2. Diagnostics Tests for HIV
1 Tests to facilitate initial diagnosis
2 Tests to stage the patients
3 Tests to monitor the patients

3. Important of HIV Testing
Known HIV status for care and treatment
For prevention measure
Problem
Poor HIV testing coverage in resource- limited Setting

4. General Community Requirement
Need to increase the level of access to high quality diagnostic in resource-limited settings
To facilitate early detection and treatment of HIV/AIDS
Simplify and improve the efficiency of HIV testing
Tests design to be delivered at The point of care (POC) or Near POC

5. The Various PCR Process have Three Steps in Common
1. Sample preparation, including viral concentration and nucleic acid extraction.
2. Amplification of the target DNA or RNA.
3. Detection of the amplified product.

6. DNA Extraction Flow with Magtration Technology

7. Automated Nucleic Acid Extraction

8. Extraction and PCR Set Up
Qiagen Symphony
Cobas Ampriprep

9. COBAS® S 201 System for Blood screening

10. Chiron Tigris for Blood screening

12. New Technologies for Viral Load Testing
Alere NAT system
disposable cartridge that provides for sample collection, cell lysis, specific target capture, reverse transcription, polymerase chain reaction amplification and real time fluorescence detection based on reporter probe hybridization on an integrated micro probe array.

14. Lab-in-a-tube Technology
The Liat System automates all testing processes
Sample preparation
Amplification
Real-time detection

15. Portable Fully Automate real-time PCR

16. The Liat™ Analyzer is developed for nucleic acid based testing and the Liat™ Tubes serve both as collection devices and as test chambers, maintaining samples in a closed system from sample collection through sample disposal. We believe that the Liat™ system is suitable for a wide range of testing labs and near-patient test settings due to its ease of use, fast turnaround time, cost effectiveness and safety features.

18. SAMBA: Simple AMplification Based Assay

21. Cepheid GeneXpert System

22. GeneXpert Cartridge and Module

24. Lab-on-Chip

27. Handy Lab

28. Microfluidic Technology

30. Nucleic acid extraction

32. QP system

37. BIST: Beads array In Single Tip

39. New HIV Drug resistant testing
Minisequencing or Single base extension

44. Bio-Strands Technology

45. Nanostring Technology

46. Hybridization
NanoString’s Technology employs two ~50 base probes per mRNA that hybridize in solution. The Reporter Probe carries the signal; the Capture Probe allows the complex to be immobilized for data collection

47. Purification and Immobilization
After hybridization, the excess probes are removed and the probe/target complexes aligned and immobilized in the nCounter Cartridge.

48. Data Collection
Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule

49. nCounter Analysis System
High level of precision
High level of sensitivity (<1 copy per cell)
No enzyme processing or Amplification
Quantification

50. Multiplexed Diagnostic
LLNL Awarded $2.5M to Move Pathogen Detection Tests to NanoString Platform
June 07, 2011
The National Institutes of Health has awarded $2.5 million to researchers at Lawrence Livermore National Laboratory to support the development of pathogen detection assays on NanoString Technologies' nCounter system.
The project aims to develop high-content, multiplex assays capable of simultaneously detecting 35 National Institute of Allergy and Infectious Diseases category A, B, and C viral agents, plus quantifying cytokine and chemokine responses in patients,

56. Science 24 June 2011: Vol. 332 no p. 1497
BIOTECHNOLOGY Lab-on-a-Chip Maker Looks to Put Hong Kong on Biotech Map

58. RMD Automation Strategy - Maximize Testing Efficiency Reduce complexity and increase automation, throughput
Complexity Today
Near-Term
Long-Term
Virology & Blood Screening
Amplicor
LC 2.0
CAP/CTM & cobas s 201
cobas s 401
cobas Amplicor
COBAS® TaqMan® 48
Other (HPV, CT/NG, Micro/Oncology)
CAP/CTM 96/48 & cobas s 201
New Generation systems
cobas 4800
cobas s 401

59. Thank you