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Clinical role of a lipid transfer protein that acts as a new apple-specific allergen  Elide A. Pastorello, MDa, Valerio Pravettoni, MDa, Laura Farioli,

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Presentation on theme: "Clinical role of a lipid transfer protein that acts as a new apple-specific allergen  Elide A. Pastorello, MDa, Valerio Pravettoni, MDa, Laura Farioli,"— Presentation transcript:

1 Clinical role of a lipid transfer protein that acts as a new apple-specific allergen 
Elide A. Pastorello, MDa, Valerio Pravettoni, MDa, Laura Farioli, BScb, Marco Ispano, MDc, Donatella Fortunato, BScd, Mara Monza, BSca, Maria Gabriella Giuffrida, BScd, Federica Rivolta, MDa, Elisabetta Scibola, MDa, Raffaella Ansaloni, MDc, Cristoforo Incorvaia, MD, Amedeo Conti, BScd, Claudio Ortolani, MDc  Journal of Allergy and Clinical Immunology  Volume 104, Issue 5, Pages (November 1999) DOI: /S (99) Copyright © 1999 Mosby, Inc. Terms and Conditions

2 Fig. 1 IgE reactivity patterns to crude apple extract in 43 patients with OAS. Patients No. 1 to 32 had birch pollinosis, No. 33 to 43 no birch pollinosis. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

3 Fig. 2 Cationic exchange of crude apple extract fractionated over HPLC Resource S (A) . Gel filtration of peak in cationic exchange chromatography (B) . Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

4 Fig. 3 SDS-PAGE staining with Coomassie brilliant blue R-250 of crude apple extract and of 9-kd purified protein and IgE immunoblotting of 9-kd purified protein. Journal of Allergy and Clinical Immunology  , DOI: ( /S (99) ) Copyright © 1999 Mosby, Inc. Terms and Conditions

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