1. Fig. 3 Agonists of innate immunity are effective only when released locally from the hydrogel.
Agonists of innate immunity are effective only when released locally from the hydrogel. (A and B) Tumors were resected from mice 10 days after orthotopic inoculation of 4T1-Luc2 cells. (A) Kaplan-Meier curves are shown for the following groups: no hydrogel, an empty hydrogel, a hydrogel loaded with R848, weekly intravenous (IV) injection of R848, weekly intraperitoneal (IP) injection of R848, or an empty hydrogel plus local administration of R848 in solution. (B) Kaplan-Meier curves are shown for the following groups: no hydrogel, an empty hydrogel, a hydrogel loaded with STING-RR, or an empty hydrogel plus local administration of STING-RR in solution. (C and D) Tumors were not resected but were instead injected intratumorally (IT) with PBS, a single dose of R848, or a single dose of STING-RR 10 days after orthotopic inoculation of 4T1-Luc2 cells. Tumor volume (C) and mouse survival (D) were measured. (E and F) Tumors were resected from mice 10 days after orthotopic inoculation of 4T1-Luc2 cells. (E) Kaplan-Meier curves are shown for the following groups: no hydrogel, a hydrogel loaded with CCL4, a hydrogel loaded with CCL5, or a hydrogel loaded with CXCL10. (F) Kaplan-Meier curves are shown for all groups described: no hydrogel, a hydrogel loaded with paclitaxel, or a hydrogel loaded with doxorubicin. The number of mice per group (n) and median survival (ms) are listed. The experiment was performed at least three times. For (A) and (B), statistics were calculated relative to the group treated with hydrogel containing R848 or STING-RR, respectively, using the log-rank (Mantel-Cox) test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ For (C) to (F), statistics were calculated relative to the no hydrogel control using the log-rank (Mantel-Cox) test, and no differences between groups were statistically significant.
Chun Gwon Park et al., Sci Transl Med 2018;10:eaar1916
Published by AAAS