1. Efficient naked plasmid cotransfection of lung grafts by extended lung/plasmid exposure time 
Franco D’Ovidio, MD, Niccolò Daddi, MD, Takashi Suda, MD, Kathleen Grapperhaus, Alexander G Patterson, MD 
The Annals of Thoracic Surgery 
Volume 71, Issue 6, Pages (June 2001)
DOI: /S (01)

2. Fig 1
Study design, Part Ia. Cold preservation versus gene cotransfection (four groups, n = 8). (CAT = chloramphenicol acetyl transferase; β-Gal = β-galactosidase; TX = transplant.)
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

3. Fig 2
Study design, Part Ib. Cold preservation versus transgene expression (three groups, n = 8). (CAT = chloramphenicol acetyl transferase; β-Gal = β-galactosidase; TX = transplant.)
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

4. Fig 3
Chloramphenicol acetyl transferase (CAT) and β-galactosidase (β-Gal) protein expression after cotransfection of lung isografts with naked plasmid pCF1-CAT and pCF1-β-Gal. Lung isografts were stored for 1, 6, and 18 hours in 4°C low potassium dextran glucose solution. CAT 1 hour group received only the pCF1-CAT and served as control toward cotransfection. Statistical significance resulted from the comparison between CAT-β-Gal 1 hour and CAT-β-Gal 18 hours for both proteins.
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

5. Fig 4
Evaluation of the impact of prolonged cold preservation on transgene expression. Chloramphenicol acetyl transferase (CAT) protein transgene expression is shown after cotransfection of lungs with naked plasmid pCF1-CAT and pCF1-β-Gal. Groups I and II No TX, no transplantation was performed and animals were sacrificed 48 hours after plasmid administration. In group III left lungs were harvested 24 hours after transfection, stored for 18 hours at 4°C, implanted and recipients were sacrificed at 24 hours. Group I-No TX received only pCF1-CAT. No statistical significant difference was observed for all comparisons, p > (β-Gal = β-galactosidase.)
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

6. Fig 5
Evaluation of naked plasmid/lung parenchyma exposure time on transfection efficiency. Chloramphenicol acetyl transferase (CAT) protein expression after 48 hours from left main bronchus selective instillation of pCF1-CAT. After instillation the left main bronchus was not clamped, clamped for 10 minutes, or clamped for 1 hour. Statistical significance resulted from comparison between 1-hour clamp versus no clamp.
The Annals of Thoracic Surgery  , DOI: ( /S (01) )

7. Fig 6
Immunohistochemistry for chloramphenicol acetyl transferase (CAT) within lungs that have undergone 1 hour of left main bronchus clamping after naked plasmid delivery. A1 shows (arrows) bronchial epithelium and peribronchial lymphocytes transfection (magnification, ×100), A2 is the negative control. B1 and C1 show (arrows) the transfection of the alveolar epithelium and of the alveolar macrophages (magnification, ×100 and ×200, respectively). B2 and C2 are the respective negative controls.
The Annals of Thoracic Surgery  , DOI: ( /S (01) )