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Proteomic analysis of externalized proteins
Proteomic analysis of externalized proteins. [35S]Methionine-labeled proteins synthesized by normal urothelium (cyst 473) or an invasive TCC (375-2 pT2-4 G4) and either maintained intracellularly (specimen) or released to the medium (released) were separated by 2D PAGE (IEF) and visualized by autoradiography. Proteomic analysis of externalized proteins. [35S]Methionine-labeled proteins synthesized by normal urothelium (cyst 473) or an invasive TCC (375-2 pT2-4 G4) and either maintained intracellularly (specimen) or released to the medium (released) were separated by 2D PAGE (IEF) and visualized by autoradiography. The area of the gel that contains the β, σ, η, and ζ isoforms is shown enlarged with the positions for the respective isoforms indicated. José M. A. Moreira et al. Mol Cell Proteomics 2004;3: © 2004 The American Society for Biochemistry and Molecular Biology
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