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The antiadhesive agent sodium hyaluronate increases the proliferation rate of human peritoneal mesothelial cells  Michel M.P.J Reijnen, M.D., Peter Falk,

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Presentation on theme: "The antiadhesive agent sodium hyaluronate increases the proliferation rate of human peritoneal mesothelial cells  Michel M.P.J Reijnen, M.D., Peter Falk,"— Presentation transcript:

1 The antiadhesive agent sodium hyaluronate increases the proliferation rate of human peritoneal mesothelial cells  Michel M.P.J Reijnen, M.D., Peter Falk, Harry van Goor, M.D., Ph.D., Lena Holmdahl, M.D., Ph.D.  Fertility and Sterility  Volume 74, Issue 1, Pages (July 2000) DOI: /S (00)

2 Figure 1 Proliferation rate, measured as optical density (at 450 nm), of nonattached human peritoneal mesothelial cells incubated with either no (open bars), 0.05% (dotted bars), 0.1% (striped bars), 0.2% (hatched bars), or 0.4% sodium hyaluronate (solid bars), 4 or 24 hours after the addition of sodium hyaluronate (n = 8 each). Controls are considered as 100%. Results are presented as mean and SEM. ∗ = P<.05,∗∗ = P<.01, ∗∗∗ = P<.005, sodium hyaluronate vs. control. Reijen. Anti-adhesive agent sodium hyaluronate. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (00) )

3 Figure 2 Proliferation rate of attached human peritoneal mesothelial cells, measured as optical density (at 450 nm), incubated with either no (open bars), 0.05% (dotted bars), 0.1% (striped bars), 0.2% (hatched bars), or 0.4% (solid bars) sodium hyaluronate, 4 or 24 hours after the addition of sodium hyaluronate (n = 8 each). Controls are considered as 100%. Results are presented as mean and SEM. ∗ = P<.05; ∗∗ = P<.01; ∗∗∗ = P<.001, sodium hyaluronate vs. control. Reijen. Anti-adhesive agent sodium hyaluronate. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (00) )

4 Figure 3 Proliferation rate of attached human peritoneal mesothelial cells treated with either LPS, TNFα, or a combination of both, and incubated with either no (open bars) or 0.2% (hatched bars) sodium hyaluronate, 4 and 24 hours after incubation (n = 8 each). Controls are considered as 100%. Results are presented as mean and SEM. ∗ = P<.05, ∗∗ = P<.001, sodium hyaluronate vs. control. + = P<.005, cells treated with proinflammatory mediators vs. untreated cells. Con = control. Reijen. Anti-adhesive agent sodium hyaluronate. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (00) )

5 Figure 4 Proliferation rate of attached human peritoneal mesothelial cells preincubated for 24 hours with either LPS, TNFα, or a combination of both, and treated with no (open bars) or 0.2% (hatched bars) sodium hyaluronate for 4 hours (n = 8 each). Controls are considered as 100%. Results are presented as mean and SEM. ∗ = P<.001, sodium hyaluronate vs. control. + = P<.05; ++ = P<.01, cells treated with proinflammatory mediators vs. untreated cells. Con = control. Reijen. Anti-adhesive agent sodium hyaluronate. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (00) )

6 Figure 5 Immunoreactivity for the CD44 receptor in human peritoneal mesothelial cells, showing receptor location at the cell surface. Staining: FITC-labeled mouse-anti-human CD44 antibody (F-3647, diluted 1:20; Sigma). Magnification, ×100. Reijen. Anti-adhesive agent sodium hyaluronate. Fertil Steril 2000. Fertility and Sterility  , DOI: ( /S (00) )


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