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Christopher W. Stamatkin, Reumen G. Roussev, Mike Stout, Carolyn B

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Presentation on theme: "Christopher W. Stamatkin, Reumen G. Roussev, Mike Stout, Carolyn B"— Presentation transcript:

1 Preimplantation factor negates embryo toxicity and promotes embryo development in culture 
Christopher W. Stamatkin, Reumen G. Roussev, Mike Stout, Carolyn B. Coulam, Elisabeth Triche, Robert A. Godke, Eytan R. Barnea  Reproductive BioMedicine Online  Volume 23, Issue 4, Pages (October 2011) DOI: /j.rbmo Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

2 Fig. 1 Synthetic preimplantation factor (sPIF) protects against toxicity of embryo toxic serum (ETS). Mouse embryos were cultured with 5% ETS and 0.78μg/ml sPIF, which increased blastocyst formation rate while reducing significantly atretic embryos as compared with ETS-only treated embryos (chi-squared=12.74, P<0.002). Table 1 details the statistical analysis of the six independent experiments. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

3 Fig. 2 High-dose exogenous synthetic preimplantation factor (sPIF) does not affect embryos cultured under optimal conditions. Embryos were cultured with 3.12μg/ml sPIF up to the blastocyst stage. Blastocyst rate of formation was slightly better than controls. Results are composite of two independent experiments. Reproductive BioMedicine Online  , DOI: ( /j.rbmo ) Copyright © 2011 Reproductive Healthcare Ltd. Terms and Conditions

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