1. C1orf106 deficiency leads disrupted cytohesin interaction with ZO-1.
C1orf106 deficiency leads disrupted cytohesin interaction with ZO-1. (A) C1ORF106 protein expression in lysates from human C1ORF106 crispr KO colon organoid generated with Cas9–guide RNA lentivirus. (B) Maximal transepithelial electrical resistance data from monolayers derived from C1ORF106 crispr KO organoid. **p < (C) Immunoprecipitation of FLAG-tagged cytohesin-1 and immunoblot with anti–ZO-1 and anti-FLAG. (D) Confocal immunofluorescence images from C1ORF106 KO monolayers immunostained with anti–cytohesin-1 and anti–ZO-1. Original magnification ×40.
Paolo Manzanillo et al. ImmunoHorizons 2018;2:
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